Product Name: Anti-CHN1 (151-165) antibody produced in rabbit
Synonym: Anti-ARHGAP2; Anti-CHN; Anti-Chimerin (chimaerin) 1; Anti-RHOGAP2
Product Type: Chemical
CAS NO: 51649-83-3Hedgehog inhibitors
antibody Form: IgG fraction of antiserum
application(s)
western blot: 1:500-1:2,000
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~53 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Anti-CHN1 (151-165) antibody is suitable for western blot at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions:
α1-chimaerin is expressed in developing neurons and is controlled by synaptic activity. It plays a crucial role in human neuronal signal-transduction mechanisms. Any alteration in α1-chimaerin expression leads to the pruning of dendritic spines and branches and supression of its expression leads to increased process growth from the dendritic shaft and from spine heads.
General description: CHN1 is a 38-kDa GTPase-activating protein (GAP) and phorbol ester receptor domain. α-chimaerin gene have two isoForms, α1and α2 chimaerin. Both the isoForms contain C1 and Rho-GAP domains but α2 isoForm contains an N-terminal SH2 domain which is absent in α1. It has N-terminal SH2 domain, the C-terminal RhoGAP domain and the central C1 domain similar to protein kinase C.
Immunogen:
synthetic peptide corresponding to amino acids 151-165 of human CHN1
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/93/3/277