Product Name: Anti-CSTF3 (C-terminal) antibody produced in rabbit
Synonym: Anti-CF-1 77 kDa subunit; Anti-CSTF-77; Anti-Cleavage stimulation factor 77
Product Type: Chemical
CAS NO: 366017-09-6Indoleamine 23-Dioxygenase (IDO) inhibitors
antibody Form: affinity isolated antibody
application(s)
immunoprecipitation: 2.5-5 μg using lysates of HEK-293T cells
indirect immunofluorescence: 1.25-2.5 μg/mL using paraFormaldehyde fixed HeLa cells
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells
biological source
rabbit
clone
polyclonal
Concentration: ~1.0 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~77 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Anti-CSTF3 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working concentration of 2.5-5 μg using lysates of HEK-293T cells, indirect immunofluorescence at 1.25-2.5 μg/mL using paraFormaldehyde fixed HeLa cells and western blotting at 0.5-1 μg/mL using lysates of HEK-293T cells.
Biochem/physiol Actions:
The cleavage stimulatory factor (CstF) is a part of the mammalian mRNA 3′ end processing complex and interacts with a less-conserved G/U-rich sequence situated downstream of the cleavage site. CSTF3 bridges CSTF1 and CSTF2 during the assembly of the CstF complex and is required for proper 3’-end cleavage.
Features and Benefits:
Antibody Bioguarantee
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General description: CstF is a heterodimer consisting of subunits of 50, 64 and 77 kDa referred to as CSTF1, CSTF2 and CSTF3, respectively. CSTF3 contains 12 HAT repeats followed by a proline-rich segment. CSTF3 also interacts directly with the RNA polymerase II CTD.
Immunogen:
synthetic peptide corresponding to amino acids 703-717 of human CSTF3, conjugated to KLH. The corresponding sequence is identical in mouse.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: nwg
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/97/2/182