Product Name: Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
MDL Number: MFCD01092314
Product Type: Chemical
CAS NO: 144-29-6Glucocorticoid Receptor inhibitors
antibody Form: IgG fraction of antiserum
application(s)
indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt 26 kDa
Shipped in: dry ice
Storage temp.: −20°C
Application: Anti-Chloramphenicol Acetyl Transferase (CAT) is suitable for use in immunoblotting and indirect immunofluorescence (10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene).
Features and Benefits:
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General description: Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT.
Immunogen:
bacterial chloramphenicol acetyl transferase (CAT).
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/95/4/421