Product Name: Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit
MDL Number: MFCD02096080
Product Type: Chemical
CAS NO: 934240-31-0EGFR inhibitors
antibody Form: affinity isolated antibody
application(s)
western blot (chemiluminescent): 1:200-1:300 using rat heart membranes and ECL.
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: lyophilized powder
species reactivity
rat
Storage temp.: −20°C
Application: Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit is suitable for western blotting (chemiluminescent) at a working dilution of 1:200-1:300 using rat heart membranes and ECL. It was used as a primary antibody for western blotting at a dilution of 1:2000 to demonstrate adenoviral-mediated overexpression of ClC-3.
Biochem/physiol Actions:
The encoded protein, voltage-gated chloride channel, is a transmembrane protein that maintains chloride ion homeostasis in various cells. It contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. ClC-3 deficiency inhibits Ang II-induced apoptosis of endothelial progenitor cells via suppressing ROS (reactive oxygen species) generation derived from NADPH oxidase. Osmotically active Cl- is extruded via this channel upon kinase activation, which facilitates cytoplasmic condensation preceding cell division. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma.
Features and Benefits:
Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perForm in your application, we will issue a full credit or replacement antibody. Learn more.
General description: The protein contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function.
Immunogen:
highly purified GST fusion protein of the rat chloride channel-3 (CLC-3) corresponding to amino acids 592-661.
Physical Form: Lyophilized at ~0.3 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose, and 0.025% sodium azide.
Specificity:
The epitope has significant homology with CLC-4 and CLC-5 proteins in rat and is well conserved in vertabrates.
Symbol
GHS05
Signal word
Danger
Hazard statements
H318
Precautionary statements
P280-P305 + P351 + P338
Hazard Codes
Xi
Risk Statements
41
Safety Statements
26-39
RIDADR: NONH for all modes of transport
WGK Germany: 2
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/96/3/213