Anti-Cofilin antibody produced in rabbit

Product Name: Anti-Cofilin antibody produced in rabbit

MDL Number: MFCD03791485
Product Type: Chemical

CAS NO: 404844-02-6Filovirus inhibitors
antibody Form: IgG fraction of antiserum
application(s)
indirect immunofluorescence: 1:1,000 using using mouse NIH/3T3 fibroblasts

microarray: suitable

western blot: 1:10,000 using using whole extracts of human A-431, rat PC-12, mouse NIH/3T3, and dog MDCK kidney cells
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~19 kDa
Shipped in: dry ice
species reactivity
human, mouse, canine, rat
Storage temp.: −20°C
Application: Anti-Cofilin antibody has been used:
•for immunostaining of chick neurons. It is used as a primary antibody
•for western blotting of protein isolated from mouse hippocampi cells, rat brain samples, human acute lymphoblastic T-cell line, human brain samples, head and neck squamous cell carcinoma cell line, HEK293T cells and renal epithelial cell lines
•for immunofluorescence studies in tissue samples from human brain

Biochem/physiol Actions:
Cofilin binds stoichiometrically to monomeric G-actin and actin protomers. Cofilin intercalates between longitudinally associated actin monomers within the filament. Under specific conditions, It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends. It is essential for viability and vital for many cellular processes involving actin remodeling, such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis etc. Cofilin activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated Form, it is inactive and unable to bond with actin. Phosphorylation of cofilin is regulated by at least four protein kinases: LIM Kinase-1, LIM Kinase-2, Testicular Kinase-1, and Testicular Kinase-2. S-nitrosylation of cofilin is involved in vascular endothelial growth factor (VEGF)-stimulated endothelial cytoskeleton remodeling and migration. Over-expression of cofilin suppresses growth and invasion of non-small cell lung cancer. Dysregulation of cofilin is associated with neurodegeneration.
Features and Benefits:
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General description: Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. In mammals it has two isoForms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells.
Immunogen:
synthetic peptide corresponding to human cofilin sequence, (amino acids 154-166) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Specificity:
Reacts with muscle and non-muscle cofilin.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/94/2/197