Product Name: Anti-Cortisol antibody produced in rabbit
Synonym: Anti-Hydrocortisone
MDL Number: MFCD00162244
Product Type: Chemical
CAS NO: 88105-29-7TGF-beta_Smad inhibitors
antibody Form: whole antiserum
application(s)
radioimmunoassay: suitable
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: lyophilized powder
Storage temp.: 2-8°C
Application: Anti-cortisol antibody is suitable for RIA. It has been used for ELISA to quantitate the levels of cortisol in the blood of fish. The antibody was also used for immunohistochemistry of trout gill filament sections at a working dilution of 1:5.
General description: Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active Form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD). Cortisol is bound to corticosteroid-binding globulin in the plasma. The organs that produce 11-HSD and involved in metabolizing and clearing it from plasma cortisol are kidney and the liver. Cortisol may be oxidised at 11-hydroxt position to result in the production of cortisone, dihydrocortisone, or tetrahydrocortisone. Cortisol and other glucocorticoids bind to the human glucocorticoid receptor (hGR) that is predominantly present in the cytoplasm. On binding, the recptor-ligand complex translocate to nucleus where the transcription of target genes is induced. Deficiency in the levels of 11-HSD are responsible for juvenile hypertension, (a feature of Apparent Mineralocorticoid Excess syndrome), edema, heart failure and hypokalemia (licorice intoxication).
Immunogen:
cortisol-21-thyroglobulin
Reconstitution:
Reconstitute with 5 mL buffer: 0.05 M Tris-HCl, pH 8.0, 0.1 M sodium chloride, 0.1% gelatin, and 15 mM sodium azide. Store this stock at −20 °C. Working dilution (1:10) to be discarded if unused within 12 hours.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: 2-8°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/93/3/329