Product Name: CAS9 Protein
Synonym: Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli
Product Type: Chemical
CAS NO: 1333210-07-3Xanthine Oxidase inhibitors
Assay: ≥90% (SDS-PAGE)
Form: lyophilized powder
Recombinant: expressed in E. coli
Shipped in: ambient
Storage temp.: −20°C
Application: Functional Genomics/Target Validation/Genome Editing
Components:
Each kit consists of:
•one vial of Cas9 recombinant protein
•one vial containing 1 mL of 1× dilution buffer
•one vial containing 1 mL of nuclease-free water with glycerol
Features and Benefits:
•Highly specific
•Highly active
•Ready-to-inject/transfect
General description: Recombinant Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, wild type Streptococcus pyogenes Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Legal InFormation: CRISPR Use License Agreement
Other Notes:
Use our CRISPR Selection Tool to order gRNA
Packaging:
pkg of 50 μg
pkg of 250 μg
Principle:
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Reconstitution:
Lyophilized S. pyogenes Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. The solution should be allowed to incubate at room temperature (20-23 °C) for 10 minutes. Centrifuge immediately before use.
RIDADR: NONH for all modes of transport
Purity: ≥90% (SDS-PAGE)
Storage Temp.: −20°C
Components
Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli; Dilution buffer for Cas9 proteins; Reconstitution solution for Cas9 proteins
PubMed ID:http://jpet.aspetjournals.org/content/97/3/314