Product Name: Calcium Phosphate Transfection Kit
EC Number: 233-140-8
Product Type: Chemical
CAS NO: 491833-30-8NEDD8-activating Enzyme inhibitors
Form: solution
grade
for molecular biology
Shipped in: dry ice
sterility
sterile; 0.2 μm filtered
Storage temp.: −20°C
usage
1 kit sufficient for 160 transfections (6 cm dishes)
1 kit sufficient for 400 transfections (3.5 cm dishes)
1 kit sufficient for 80 transfections (10 cm dishes)
Application: Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
Components:
The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
Features and Benefits:
•Suitable for transient and stable transfection
•Reproducible for a wide range of cell types
•Widely referenced
•Inexpensive
Frequently Asked Questions:
Frequently Asked Questions are available for this Product.
General description: Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
Other Notes:
The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
Principle:
The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate Forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.
Symbol
GHS07
Signal word
Warning
Hazard statements
H319
Precautionary statements
P305 + P351 + P338
Hazard Codes
Xi
Risk Statements
36
Safety Statements
22-24
RIDADR: NONH for all modes of transport
Storage Temp.: −20°C
UNSPSC
12352200
PubMed ID:http://jpet.aspetjournals.org/content/97/2/229