Product Name: Caspase 3 Assay Kit, Colorimetric
Product Type: Chemical
CAS NO: 36051-31-7Amyloid-(beta) inhibitors
Quality Level
PREMIUM
Shipped in: dry ice
Storage temp.: −20°C
usage
sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
sufficient for 100 standard tests (1 ml)
Application: The assay can be perFormed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.
General description: Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
Other Notes:
Use 17 megohm water only
Unit Definition:
One unit of caspase 3 will cleave 1.0μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.
Symbol
GHS06, GHS08
Signal word
Danger
Hazard statements
H301-H311-H331-H373-H412
Precautionary statements
P261-P273-P280-P301 + P310-P311
Hazard Codes
T
Risk Statements
23/24/25-33-52/53
Safety Statements
36/37-45-61
RIDADR: UN 3316 9
Flash Point(F)
415.4 °F
Flash Point(C)
213 °C
Storage Temp.: −20°C
UNSPSC
12352200
Components
Ac-DEVD-CHO inhibitor .5 mg; Ac-DEVD-pNA substrate 15 mg; 10X Assay buffer 20 mL; Caspase 3 positive control 5 μg; p-Nitroaniline standard 1 mg; Water (17-18 megaOhm) 125 mL
PubMed ID:http://jpet.aspetjournals.org/content/97/3/331