Product Name: Caspase 3 Assay Kit, Fluorimetric
EC Number: 247-454-8
Product Type: Chemical
CAS NO: 54197-31-8CaMK inhibitors
Shipped in: dry ice
Storage temp.: −20°C
usage
sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)
Application: The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.
General description: The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).
Unit Definition:
One unit of caspase3 will cleave 1.0μmol of Ac-DEVD per min at pH7.4 at 25 °C.
Symbol
GHS07
Signal word
Warning
Hazard statements
H315-H319-H335
Precautionary statements
P261-P305 + P351 + P338
Hazard Codes
Xi
Risk Statements
36/37/38
Safety Statements
26-36
RIDADR: NONH for all modes of transport
Storage Temp.: −20°C
UNSPSC
12352200
Components
Ac-DEVD-AMC substrate 2.5 mg; Ac-DEVD-CHO inhibitor .5 mg; 7-Amino-4-methylcoumarin standard 1 mg; 10X Assay buffer 20 mL; Caspase 3 positive control 5 μg; 5X Lysis buffer 5 mL; Water (17-18 megaOhm) 125 mL
PubMed ID:http://jpet.aspetjournals.org/content/97/3/342