Caspase 8 Assay Kit, Colorimetric

Product Name: Caspase 8 Assay Kit, Colorimetric

Product Type: Chemical

CAS NO: 585531-74-4Serotonin Transporter inhibitors
Shipped in: dry ice
Storage temp.: −20°C
Application: The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
•purified caspase 8 for a positive control
•caspase 8 inhibitor
•p-NA fluorescent standard

Quantity:
The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.
RIDADR: NONH for all modes of transport
Storage Temp.: −20°C
UNSPSC
12352200
Components
Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor 100 μL; Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL; Water, 17-18 megohm 125 mL; 10X Assay Buffer 5 mL; 5X Lysis Buffer 5 mL
PubMed ID:http://jpet.aspetjournals.org/content/97/3/349