Product Name: Caspase 9 human
Synonym: ICE-Lap6; Mch6
MDL Number: MFCD02687006
Product Type: Chemical
CAS NO: 1627676-59-8Anti-infection inhibitors
Assay: ≥90% (SDS-PAGE)
Form: buffered aqueous solution
Mol wt: N-terminal prodomain plus the large subunit. mol wt 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein)
small subunit mol wt 13 kDa (subunit containing the histidine tag)
Recombinant: expressed in E. coli (C-terminal histidine-tagged)
Shipped in: dry ice
Storage temp.: −70°C
Biochem/physiol Actions:
When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP.
Packaging:
25 μg in glass bottle
Physical Form: Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.
Unit Definition:
One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.
RIDADR: NONH for all modes of transport
WGK Germany: 2
Purity: ≥90% (SDS-PAGE)
Storage Temp.: −70°C
UNSPSC
12352204
PubMed ID:http://jpet.aspetjournals.org/content/94/2/178