Product Name: Catalase Assay Kit
Product Type: Chemical
CAS NO: 1260075-17-9NF-κB inhibitors
Shipped in: wet ice
Storage temp.: 2-8°C
usage
sufficient for ≥100 tests enzymatic
Application: Sutitable for Colorimetric and UV Assays
Biochem/physiol Actions:
Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is Formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.
Features and Benefits:
•Useful for determining catalase activity – may be used in various tissues and cells
•Simple, optimized protocol – A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity
General description: The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.
Preparation Note: Use ultrapure water in preparation of all solutions.
Unit Definition:
One unit of catalase will decompose 1.0micromole of hydrogen peroxide to oxygen and water per minute at pH7.0 at 25°C at a substrate concentration of 50mM hydrogen peroxide.
RIDADR: NONH for all modes of transport
WGK Germany: 1
Storage Temp.: 2-8°C
UNSPSC
12352200
Components
Assay Buffer 10X 100 mL; Chromagen Reagent 1 vial; Stop Solution 100 mL; Catalase Positive Control .25 mL; Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS) 5 mg; Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
PubMed ID:http://jpet.aspetjournals.org/content/97/3/358