Product Name: Cathepsin C from bovine spleen
Synonym: Dipeptidyl aminopeptidase; Dipeptidyl peptidase I
CAS Number: 9032-68-2
EC Number: 232-881-4
MDL Number: MFCD00081490
Product Type: Chemical
CAS NO: 1313613-09-0screening-libraries inhibitors
composition
Protein, ≥25% biuret
Form: lyophilized powder
Shipped in: dry ice
Storage temp.: −20°C
type
Type X
Application: Cathepsin C has been used in a study that demonstrated the potential of a proteomics approach to identify novel proteins expressed by extravillous trophoblast and to uncover the mechanisms leading to disease states in pregnancy. Cathepsin C has also been used in a study to evaluate biodegradable thermogels.
Application: The enzyme from Sigma has been used in the activation of granzyme k (Gzmk) precursor from E. coli. Granzymes are granule-stored lymphocyte serine proteases that are implicated in T- and natural killer cell-mediated cytotoxic defense reactions.
Biochem/physiol Actions:
Cathespin C is a dipeptidyl aminopeptidase that can sequentially remove dipeptides from a peptide chain with an unsubstituted N-terminus. The enzyme exhibits a preference for glycine and proline as N-terminal aminoacids. Substrates that have an N-terminal lysyl or arginyl residue, or a penultimate proryl residue are not targeted by this enzyme. The endopeptidase activity requires the presence of halide ions and sulfydryl activators.
Caution:
Unstable. Keep frozen.
Packaging:
10, 25, 100 units in glass bottle
Physical Form: Lyophilized from a 1 M sodium chloride solution.
Unit Definition:
One unit will produce 1μmole of Gly-Phe-NHOH from Gly-Phe-NH2 and hydroxylamine per min at pH6.8 at 37°C using
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −20°C
Enzyme Commission (EC) Number
3.4.14.1(BRENDA|IUBMB)
UNSPSC
12352204
PubMed ID:http://jpet.aspetjournals.org/content/93/3/423