Product Name: CelLytic™ B Plus Kit
EC Number: 200-664-3
Product Type: Chemical
CAS NO: 39262-14-1Keap1-Nrf2 inhibitors
Shipped in: wet ice
Storage temp.: −20°C
usage
1 kit sufficient for 5 g fresh or frozen cell paste lysis.
Application: This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chromatography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.
Features and Benefits:
•Lyse Gram positive and Gram negative bacteria
•More efficient than sonication
•Compatible with affinity purification
•Isolate inclusion bodies for subsequent solubilization
•Non-denaturing cell lysis preserves protein function
Frequently Asked Questions:
Frequently Asked Questions are available for this Product.
General description: The CelLytic™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.
Legal InFormation: Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal InFormation: Benzonase® Nuclease is manufactured by Merck KGgA and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Legal InFormation: CelLytic is a trademark of Sigma-Aldrich Co. LLC
Legal InFormation: Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Legal InFormation: FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Legal InFormation: HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes:
The CelLytic™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.
Symbol
GHS07
Signal word
Warning
Hazard statements
H315-H319
Precautionary statements
P305 + P351 + P338
Risk Statements
36/38
Safety Statements
24-26
RIDADR: NA 1993 / PGIII
Storage Temp.: −20°C
UNSPSC
12352200
Components
CelLytic™ B Cell Lysis Reagent, For bacterial cell lysis, standard strength; Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution; Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution; Lysozyme from chicken egg white, 10 mg/mL
PubMed ID:http://jpet.aspetjournals.org/content/97/3/371