Product Name: Checkpoint Kinase 2, Active human
Synonym: S. pombe homolog; Chek2; Chk2
MDL Number: MFCD07370672
Product Type: Chemical
CAS NO: 1246819-17-9Cell Cycle_DNA Damage inhibitors
Assay: ≥85% (SDS-PAGE)
Form: buffered aqueous glycerol solution
Mol wt: protein apparent mol wt ~88 kDa
Recombinant: expressed in E. coli
Shipped in: dry ice
Storage temp.: −70°C
Application: Kinase activity is measured as the molar amount of phosphate incorporated into the CHKtide per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
Biochem/physiol Actions:
Chk2 is the mammalian homolog of the S.cerevisiae Rad53 and S.pombe Cds1 protein kinase required for DNA damage and replication checkpoints. Chk2 is rapidly activated, by phosphorylation, in response to replication blocks and DNA damage.
Physical Form: Supplied at a concentration of approximately 0.1 mg/mL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol
RIDADR: NONH for all modes of transport
WGK Germany: 1
Purity: ≥85% (SDS-PAGE)
Storage Temp.: −70°C
UNSPSC
12352200
PubMed ID:http://jpet.aspetjournals.org/content/94/4/382