Product Name: Monoclonal Anti-Cathepsin K antibody produced in mouse
Synonym: Anti-CTSK; Anti-CTSO2; Anti-PKND; Anti-PYCD
MDL Number: MFCD02097890
Product Type: Chemical
CAS NO: 1639042-08-2NF-(kappa)B inhibitors
antibody Form: purified immunoglobulin
application(s)
indirect ELISA: suitable
western blot: 10-20 μg/mL using total cell extracts of HCT-116 cells
biological source
mouse
clone
CL124-1H6, monoclonal
Concentration: ~2 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
isotype
IgG2b
Mol wt: antigen mol wt ~27 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Anti-Cathepsin K antibody was used as primary antibody in western blotting of spine extracts. It is also suitable for indirect ELISA.
Biochem/physiol Actions:
Cathepsin is involved in various functions such as intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Among all the types, cathepsins K (Ctsk) is highly expressed in osteoclasts that plays a crucial role in bone remodeling and resorption. Cathepsin K protein was found in primary breast carcinoma and prostate cancer where it perForms in the invasive process of prostate cancers, thyroid carcinoma, lung adenocarcinoma and breast carcinoma. Its deficency causes pycnodysostosis, a rare bone dysplasia with an osteopetrotic-like phenotype.
General description: Cathepsins are proteases that normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell. Cathepsins are classified based on their functions and presence of active site such as cathepsin B, D, K, V, F etc.
Immunogen:
recombinant human cathepsin K. The antibody epitope resides within the peptide sequence RGYREIPEGNEKAL that corresponds to amino acids 222-235.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Specificity:
The antibody does not cross react with the closely related lysosomal cysteine proteases cathepsins L, B, H, S, V and W.
RIDADR: NONH for all modes of transport
WGK Germany: 2
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/93/1/93