Product Name: Monoclonal Anti-Chk2 antibody produced in mouse
MDL Number: MFCD03457550
Product Type: Chemical
CAS NO: 1454682-72-4ROCK inhibitors
antibody Form: purified immunoglobulin
application(s)
immunohistochemistry (Formalin-fixed, paraffin-embedded sections): suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
biological source
mouse
clone
DCS-273, monoclonal
Concentration: ~2 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
isotype
IgG1
Mol wt: antigen mol wt 61 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunohistochemistry (Formalin-fixed, paraffin-embedded sections) and for immunoblotting at a concentration of 2 to 4 μg/mL using whole extract of cultured 293T cells.
Biochem/physiol Actions:
The protein propagates signals from damaged or unreplicated DNA. The protein is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
Features and Benefits:
Antibody Bioguarantee
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Immunogen:
recombinant human Chk2.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Specificity:
The epitope recognized by the antibody resides within the FHA domain of the Chk2 molecule. This epitope is likely to be masked by protein-protein interactions in vivo.
WGK Germany: 2
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/95/2/122