S earlier described [17,18]. For MCF-7/AZ cell line, due to its lower expression of P-cadherin, 50 mg of total protein lysate has been loaded; for BT-20, due to its P-cadherin overexpression, the gel loading was done only with 20 mg of protein lysate. Membranes were incubated with primary antibodies according to the conditions described in …
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