Minutes. The supernatant was discarded and also the pellet resuspended in buffer A (50 mM Tris, 2 mM EDTA, 5 mM MgCl2 at pH 7.0) and incubated at 37 for ten minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Soon after resuspending the pellet in buffer A, the suspension was …

Minutes. The supernatant was discarded plus the pellet resuspended in buffer A (50 mM Tris, 2 mM EDTA, 5 mM MgCl2 at pH 7.0) and incubated at 37 for ten minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Just after resuspending the pellet in buffer A, the suspension was incubated …

Minutes. The supernatant was discarded along with the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for 10 minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. After resuspending the pellet in buffer A, the suspension was incubated …

Minutes. The supernatant was discarded and the pellet resuspended in buffer A (50 mM Tris, 2 mM EDTA, 5 mM MgCl2 at pH 7.0) and incubated at 37 for 10 minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Just after resuspending the pellet in buffer A, the suspension was incubated …

Minutes. The supernatant was discarded along with the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for 10 minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Soon after resuspending the pellet in buffer A, the suspension was …

Minutes. The supernatant was discarded along with the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for ten minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Following resuspending the pellet in buffer A, the suspension was incubated …

Minutes. The supernatant was discarded plus the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for ten minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. After resuspending the pellet in buffer A, the suspension was incubated for …

Minutes. The supernatant was discarded as well as the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for ten minutes. Following the incubation, the suspension was centrifuged for 20 minutes at 23,000g. Soon after resuspending the pellet in buffer A, the suspension …

Te pictures to define numerical classes able to describe the unique target objects composing the image layout. The second (i.e., classification) analyzed the source photos, employing the numerical classes defined in the prior module, to supply a classification from the distinct image zones. Lastly, the final (i.e., segmentation) defined the boundaries in between heterogeneous zones …

Te images to define numerical classes in a position to describe the distinct target objects composing the image layout. The second (i.e., classification) analyzed the source pictures, applying the numerical classes defined in the earlier module, to provide a classification on the different image zones. Ultimately, the last (i.e., segmentation) defined the boundaries among heterogeneous …