Name :
Recombinant Human PMVK Protein (His Tag)
Biological Activity :
Background :
PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
Q15126
NCBI Gene ID :
Synonyms :
Synonyms :
phosphomevalonate kinase
Amino Acid Sequence :
Molecular Weight :
The recombinant human PMVK consists of 207 amino acids and predicts a molecular mass of 23.8 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the mature form of human PMVK(Q15126) (Met1-Leu192) was expressed with a polyhistidine tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM MOPS, 150mM KCl, 1mM DTT, pH 7.0.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
HUMPMKI Protein, Human; PMK Protein, Human; PMKA Protein, Human; PMKASE Protein, Human PMVK 背景信息 PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.
References & Citations :
Chambliss K.L., et al.,(1996), Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence. J. Biol. Chem. 271:17330-17334.Olivier L.M., et al., (1999), Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting.J. Lipid Res. 40:672-679.Herdendorf T.J., et al.,(2006), Phosphomevalonate kinase: functional investigation of the recombinant human enzyme. Biochemistry 45:3235-3242.
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