Name :
Recombinant Human PVRIG Protein (mFc Tag), HPLC-verified
Biological Activity :
Background :
Biological Activity :
1.Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2 His(Cat:10005-H08H) at 2 μg/mL (100 μL/well) can bind Human PVRIG mFc(Cat:28312-H38H2), the EC50 of Human PVRIG mFc(Cat:28312-H38H2) is 40-260 ng/mL.2.Loaded Human PVRIG Protein, mFc Tag(Cat.No.28312-H38H2) on AMC Biosensor, can bind Human Nectin-2 protein (Cat.No.10005-HCCH) with an affinity constant of 0.680μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).3.Loaded Human PVRIG Protein, mFc Tag(Cat.No.28312-H38H2)on AMC Biosensor, can bind Human Nectin-2 protein, hFc Tag (Cat.No.10005-H02H) with an affinity constant of 8.784nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q6DKI7-1
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant human PVRIG consists of 365 amino acids and predicts a molecular mass of 40.15 kDa. It migrates as an approximately 52.66 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human PVRIG (Q6DKI7-1) (Thr41-Leu172) was expressed with the Fc region of mouse IgG2a at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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