Name :
Recombinant Human SOX2 Protein (His Tag)
Biological Activity :
Background :
SOX2 is a member of the SOXB1 subfamily of transcription factors most notably involved in the reprogramming of somatic cells into induced pluripotent stem cells. Housed on chromosome 3, the SOX2 gene encodes for a protein with a C-terminal transcriptional activation domain and a DNA-binding HMG domain that contains a nuclear localization/export signal. SOX2 forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development and pluripotency such as YES1, FGF4, UTF1 and ZFP206. Mutations in this gene have been associated with bilateral eye disease. SOX2 antigen and antibody were found in small-cell lung cancer cell lines and sera. However, neither exists in normal sera, suggesting that SOX2 might be a potential tumor target or marker.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P48431
NCBI Gene ID :
Synonyms :
Synonyms :
SRY (sex determining region Y)-box 2
Amino Acid Sequence :
Molecular Weight :
The recombinant Human SOX2 consists of 324 amino acids and predicts a molecular mass of 35.27 kDa.
Purity :
≥ 80 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human SOX2 (P48431) (Met1-Met317) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Supplied as sterile PBS, 1mM EDTA, 1M Urea, 1mM TCEP, pH 7.4.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ANOP3 Protein, Human; MCOPS3 Protein, Human SOX2 背景信息 SOX2 is a member of the SOXB1 subfamily of transcription factors most notably involved in the reprogramming of somatic cells into induced pluripotent stem cells. Housed on chromosome 3, the SOX2 gene encodes for a protein with a C-terminal transcriptional activation domain and a DNA-binding HMG domain that contains a nuclear localization/export signal. SOX2 forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development and pluripotency such as YES1, FGF4, UTF1 and ZFP206. Mutations in this gene have been associated with bilateral eye disease. SOX2 antigen and antibody were found in small-cell lung cancer cell lines and sera. However, neither exists in normal sera, suggesting that SOX2 might be a potential tumor target or marker.
References & Citations :
1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006. doi: 10.1016/j.cell.2006.07.024.2. Kelberman D, et al. Mutations within Sox2/SOX2 are associated with abnormalities in the hypothalamo-pituitary-gonadal axis in mice and humans. J Clin Invest. 2006. doi: 10.1172/JCI28658.3. Chuang HM, et al. SOX2 for Stem Cell Therapy and Medical Use: Pros or Cons? Cell Transplant. 2020. doi: 10.1177/0963689720907565.
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