Name :
Recombinant Human NR1A2/THRB Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Thyroid Hormone Receptor beta (THRB), also known as TRbeta and NR1A2, belongs to the nuclear hormone receptor family. Featuring an N-terminal transactivation domain, a central DNA-binding domain, and a hormone/ligand-binding domain, THRB isoform 1/2 expression localizes predominately to the liver and pituitary, respectively. THRB regulates the feedback of T3-dependent thyrotropin-stimulating hormone (TSH) transcription in the pituitary by binding to thyroid hormone (TH) response elements present on promoters of TH-responsive genes. Located on chromosome 3p24, THRB gene mutation has been linked to autosomal dominant resistance to TH (RTH), which is characterized by goiter and elevated levels of T3, T4, and TSH. THRB is a known pharmacological target for the treatment of non-alcoholic steatohepatitis (NASH). THRB may be a target for future cancer therapies since its knockdown enhanced the viability of endometrial cancer cells and activated the mTOR-4EBP1/eIF4G pathway.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P10828-1
NCBI Gene ID :
Synonyms :
Synonyms :
Thyroid Hormone Receptor, beta
Amino Acid Sequence :
Molecular Weight :
The recombinant Human THRB consists of 267 amino acids and predicts a molecular mass of 30.33 kDa.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human THRB (P10828-1) (Glu202-Asp461) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 1mm Tcep, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
C-ERBA-2 Protein, Human; C-ERBA-BETA Protein, Human; ERBA2 Protein, Human; GRTH Protein, Human; NR1A2 Protein, Human; PRTH Protein, Human; THR1 Protein, Human; THRB1 Protein, Human; THRB2 Protein, Human NR1A2/THRB 背景信息 Thyroid Hormone Receptor beta (THRB), also known as TRbeta and NR1A2, belongs to the nuclear hormone receptor family. Featuring an N-terminal transactivation domain, a central DNA-binding domain, and a hormone/ligand-binding domain, THRB isoform 1/2 expression localizes predominately to the liver and pituitary, respectively. THRB regulates the feedback of T3-dependent thyrotropin-stimulating hormone (TSH) transcription in the pituitary by binding to thyroid hormone (TH) response elements present on promoters of TH-responsive genes. Located on chromosome 3p24, THRB gene mutation has been linked to autosomal dominant resistance to TH (RTH), which is characterized by goiter and elevated levels of T3, T4, and TSH. THRB is a known pharmacological target for the treatment of non-alcoholic steatohepatitis (NASH). THRB may be a target for future cancer therapies since its knockdown enhanced the viability of endometrial cancer cells and activated the mTOR-4EBP1/eIF4G pathway.
References & Citations :
1.T. Parry, D. Ledee, M.S. Willis, M.A. Portman, Chapter 10 – Nuclear Receptors and the Adaptive Response of the Heart, Editor(s): Jonathan C. Schisler, Charles H. Lang, Monte S. Willis, Endocrinology of the Heart in Health and Disease, Academic Press, 2017,2.Lai HP, Chen MH. Atrial Fibrillation with Heart Failure in a Case with Resistance to Thyroid Hormone Due to a Rare Thyroid Hormone Receptor β Gene Mutation. Int J Mol Sci. 2022.3.Ke S, et al. Thyroid Hormone Receptor β sumoylation is required for thyrotropin regulation and thyroid hormone production. JCI Insight. 2021.4.Krystian Jazdzewski, et al. Thyroid Hormone Receptor β (THRB) Is a Major Target Gene for MicroRNAs Deregulated in Papillary Thyroid Carcinoma (PTC), The Journal of Clinical Endocrinology & Metabolism, 2011.5.Ren B, et al. Thyroid Hormone Receptor β Knockdown Reduces Suppression of Progestins by Activating the mTOR Pathway in Endometrial Cancer Cells. International Journal of Molecular Sciences. 2022.
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