Name :
Recombinant Human TLK1 Protein (His & GST Tag)
Biological Activity :
Background :
Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. TLK1 has a function important for proper chromosome segregation and maintenance of diploid cells at mitosis in mammalian cells that could be mediated by reduced phosphorylation of histone H3 and condensation of chromosomes, although other explanations to the phenotype are possible.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
Q9UKI8-1
NCBI Gene ID :
Synonyms :
Synonyms :
tousled like kinase 1
Amino Acid Sequence :
Molecular Weight :
The recombinant human TLK1/GST chimera consists of 1003 amino acids and has a calculated molecular mass of 114 kDa. It migrates as an approximately 125 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 88 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human TLK1 isoform 1 (Q9UKI8-1) (Met 1-Tyr 766) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Buffer Solution :
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
KIAA0137 Protein, Human; PKU-beta Protein, Human TLK1 背景信息 Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. TLK1 has a function important for proper chromosome segregation and maintenance of diploid cells at mitosis in mammalian cells that could be mediated by reduced phosphorylation of histone H3 and condensation of chromosomes, although other explanations to the phenotype are possible.
References & Citations :
Sillje H.H.W., et al., 2001, Curr. Biol. 11:1068-73. Beausoleil S.A., et al., 2004, Proc. Natl. Acad. Sci. USA. 101:12130-5. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64. Mayya V., et al., 2009, Sci. Signal. 2: RA46-RA46.
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