Name :
Recombinant Human Tryptophan Hydroxylase 1/TPH1 Protein (His Tag)
Biological Activity :
Background :
Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.
Biological Activity :
Measured by its ability to catalyze Trp hydroxylation to 5-HTP,The specific activity is >10 pmol/min/μg.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P17752-1
NCBI Gene ID :
Synonyms :
Synonyms :
tryptophan hydroxylase 1
Amino Acid Sequence :
Molecular Weight :
The recombinant human TPH1 consisting of 458 amino acids and has a calculated molecular mass of 52.7 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human TPH1 (P17752-1) (Ile 2-Ile 444) was expressed, with a polyhistide tag at the N-terminus.
Buffer Solution :
Supplied as sterile 20mM Tris, 200mM NaCl, 10% glycerol, pH 8.0Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
TPRH Protein, Human; TRPH Protein, Human Tryptophan Hydroxylase 1/TPH1 背景信息 Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.
References & Citations :
Antonia S. New, et al.,1998, American Journal of Medical Genetics Part B. 81 (1): 13-17. Y. C Wang, et al.,2001, Neuropsychobiology. 43 (1): 1 – 4. Diego J. et al., 2003, Science. 299 (5603): 76. Allen NC, et al.,2008, Nat Genet. 40 (7): 827-34. Galfalvy,H. et al., 2009, J Affect Disord. 115 (3):331-8.
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