Name :
Recombinant Human TRIB2 Protein (His & GST Tag)
Biological Activity :
Background :
Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate the activation of some intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles control the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_067675.1
NCBI Gene ID :
Synonyms :
Synonyms :
tribbles pseudokinase 2
Amino Acid Sequence :
Molecular Weight :
The recombinant human TRIB2/GST chimera consists of 578 amino acids and predicts a molecular mass of 66 kDa.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human TRIB2 (NP_067675.1) (Met 1-Asn 343) was fused with the GST tag at the N-terminus and a polyhistidine tag at the C-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 100mM NaCl, 1mM GSH, 0.5mM PMSF, pH 8.0Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
C5FW Protein, Human; FLJ57420 Protein, Human; GS3955 Protein, Human; TRB2 Protein, Human; TRIB2 Protein, Human; TRIBBLE Protein, Human TRIB2 背景信息 Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate the activation of some intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles control the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).
References & Citations :
Seher, TC. et al., 2000, Curr Biol. 10 (11): 623-9. Keeshan, K. et al., 2006, Cancer Cell. 10 (5): 401-11. Hegedus, Z. et al., 2007, Cell Signal. 19 (2):238-50. Keeshan, K. et al., 2008, Blood Cells Mol Dis. 40 (1): 119-21. Cvetkovic, LV. et al., 2010, J Clin Invest. 120 (3): 713-9.
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