Name :
Recombinant Human TSLP Protein (hFc Tag)
Biological Activity :
Background :
Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like cytokine originally characterized by its ability to promote the activation of B cells and dendritic cells (DCs). Thymic stromal lymphopoietin (TSLP) is a cytokine expressed by epithelial cells, including keratinocytes, and is important in allergic inflammation. Subsequent studies have shown that TSLP promotes T helper type 2 (TH2) cell responses associated with immunity to some helminth parasites and the pathogenesis of many inflammatory diseases, including atopic dermatitis and asthma. TSLP can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of DC-derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues.
Biological Activity :
Immobilized Recombinant Human CRLF2 Protein (His Tag)(Cat:29749-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TSLP Protein (R127A, R130S, Fc Tag)(Cat:16135-H02H), the EC50 is 1.5-4.0 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_149024.1
NCBI Gene ID :
Synonyms :
Synonyms :
thymic stromal lymphopoietin
Amino Acid Sequence :
Molecular Weight :
The recombinant Human TSLP consists of 369 amino acids and predicts a molecular mass of 41.50 kDa. It migrates as an approximately 46.22 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human TSLP (NP_149024.1) (R127A, R130S, Met1-Gln159) was expressed with the fused Fc region of human IgG1 at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Comeau MR, et al. (2010) The influence of TSLP on the allergic response. Mucosal Immunol. 3 (2): 138-47.Ziegler SF. (2010) The role of thymic stromal lymphopoietin (TSLP) in allergic disorders. Curr Opin Immunol. 22 (6): 795-9.Liu YJ, et al. (2007) TSLP: An Epithelial Cell Cytokine that Regulates T Cell Differentiation by Conditioning Dendritic Cell Maturation. Annual Review of Immunology. 25: 193-219.He R, et al. (2008) TSLP acts on infiltrating effector T cells to drive allergic skin inflammation.PNAS. 105 (33): 11875-80.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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