Product Name: Anti-CHN2 (16-30) antibody produced in rabbit
Synonym: Anti-β-Chimaerin; Anti-ARHGAP3; Anti-BCH; Anti-RHOGAP3
Product Type: Chemical
CAS NO: 844882-93-5Metabolic Disease inhibitors
antibody Form: IgG fraction of antiserum
application(s)
western blot: 1:500-1:2,000
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~54 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Anti-CHN2 (16-30) antibody produced in rabbit is suitable for western blot at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions:
GTPase-activating protein activity of CHN2 is regulated by binding of phospholipid and diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane.
General description: α-chimaerin, a GTPase-activating protein gene have two isoForms, α1- and α2-chimaerin. Both the isoForms contain C1 and Rho-GAP domains. But α2 (β) isoForm contains an unique N-terminal SH2 domain whereas it is absent in α1. It has a cysteine-rich phorbol ester binding domain. It is expressed in granule cells, specifically in late stage spermatocytes. It has molecular mass of 30-kDa.
Immunogen:
synthetic peptide corresponding to amino acids 16-30 of human CHN2
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 2
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/93/3/414