Product Name: Chymopapain from papaya latex
CAS Number: 9001-09-6
EC Number: 232-580-8
MDL Number: MFCD00130801
Product Type: Chemical
CAS NO: 1395048-49-3Anti-cancer_Compound_Library inhibitors
composition
Protein, ≥75% E
Form: lyophilized powder
solubility
H2O: soluble5.0 mg/mL, clear, colorless
Storage temp.: −20°C
Application: Chymopapain from papaya latex has been used in a study to assess the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides. Chymopapain from papaya latex has also been used in a study to investigate the preparation and identification of the rabbit′s antibody of chymopapain.
Application: The enzyme has been used to detach cultured CD34+ cells from beads (130 units/mL for 2 minutes, repeated three times). Chymopapain and pronase have been found to be effective in the digestion of human intestinal tissue. The enzyme has been used along with pronase, collagenase, elastase, DNAse, and catalase for rabbit lung cells digestion at a concentration of 0.05% in calcium-magnesium-free Kreb′s serum substitute. It has also been used for the release of neuroblastoma cells from marrow bound to antibody-coated microspheres.
Biochem/physiol Actions:
Plant cysteine proteinase that is similar in function to lysosomal cathepsin L.
Biochem/physiol Actions:
The enzyme cleaves amide linkages at the carboxyl side of arginine, leucine, glutamic acid, and alanine residues; as well as that of arginine esters. The optimum pH varies depending on the kind of substrate. For example, the pH optimum for casein digestion is 7.9, hemoglobin digestion is 4.0 and urea-denatured hemoglobin digestion is 7.0. Molecular mass of the enzyme is found to be 27 KDa.
Other Notes:
Contains
Preparation Note: Chromatographically purified
Unit Definition:
One unit will hydrolyze 1.0 μmole of BAEE to Nα-benzoyl-
Symbol
GHS07, GHS08
Signal word
Danger
Hazard statements
H315-H317-H319-H334-H335
Precautionary statements
P261-P280-P305 + P351 + P338-P342 + P311
Hazard Codes
Xn
Risk Statements
36/37/38-42/43
Safety Statements
23-26-36/37-45
RIDADR: NONH for all modes of transport
WGK Germany: 3
RTECS
GC2800000
Storage Temp.: −20°C
Enzyme Commission (EC) Number
3.4.22.6(BRENDA|IUBMB)
UNSPSC
12352204
PubMed ID:http://jpet.aspetjournals.org/content/93/3/430