Product Name: Anti-CHIP (C-terminal) antibody produced in rabbit
Synonym: Anti-CHIP, carboxy terminus of Hsp70p-interacting protein; Anti-HSPABP2; Anti-NY-CO-7; Anti-STUB1, stip2 homology and U-box containing protein 1; Anti-UBOX1
Product Type: Chemical
CAS NO: 1246820-62-1Bombesin Receptor inhibitors
antibody Form: affinity isolated antibody
application(s)
western blot: 1-2 μg/test using HEK-293T cells lysate and mouse brain extract (S2 fraction)
biological source
rabbit
clone
polyclonal
Concentration: ~1 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~35 kDa
Shipped in: dry ice
species reactivity
mouse, human, rat
Storage temp.: −20°C
Application: Anti-CHIP (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2 μg/mL using HEK-293T cells lysate and mouse brain extract (S2 fraction).
Biochem/physiol Actions:
CHIP is a dual-function chaperone/E3 ubiquitin ligase that plays a crucial role in regulating protein quality control at multiple levels. It is comprised of a N-terminal tetratricopeptide repeat (TPR), a U-box at its C-terminus and a highly charged internal region. It ubiquitinates Hsp70, a cytosolic chaperone and enhances refolding of stress damaged proteins. Additionally, it has E3 ubiquitin ligase activity and triggers proteasome degradation of irreversibly damaged proteins to prevent cellular toxicity. The ubiquitination, aggregation and degradation of several proteins involved in neurodegenerative disorders including tau, huntingtin, Cu/Zn SOD1, ataxin-1, and α-synuclein are regulated by CHIP and Hsp70. The aggregation and toxicity of polyglutamine (polyQ) expanded proteins is suppressed by CHIP. It also regulates stress-dependent apoptosis.
Features and Benefits:
Antibody Bioguarantee
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Immunogen:
synthetic peptide corresponding to amino acids 251-268 of human CHIP conjugated to KLH. This sequence is identical in mouse and rat CHIP.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/95/3/318