Product Name: Anti-Cullin-4B antibody produced in rabbit
Synonym: Anti-Cul4B; Anti-MRXHF2; Anti-MRXSC; Anti-SFM2
Product Type: Chemical
CAS NO: 1375105-96-6Hexokinase inhibitors
antibody Form: affinity isolated antibody
application(s)
western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B.
biological source
rabbit
clone
polyclonal
Concentration: ~1.0 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt ~100 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Antigen Background:
Cullin-4B Forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators
Application: Anti-Cullin-4B antibody produced in rabbit is suitable for western blotting at a working concentration of 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. The antibody was used for immunoblotting in a study to demonstrate that CUL4B E3 ubiquitin ligase plays a key role in targeting Jab1 (Jun activation domain-binding protein) for degradation and regulates bone morphogenetic protein signaling.
Biochem/physiol Actions:
Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases that regulate cell cycle, genome stability and DNA replication. Cul4B functions as an E3 ligase involved in selecting specific targets for ubiquitylation. It associates with damage-specific DNA binding proteins DDB1 and DDB2 (UV-damaged DNA) and functions as a substrate-recruiting factor for Cul4B E3 ligases. Cul4B ubiquitylates several target proteins including β-catenin, cyclin E, and histone H2A Mutations in this gene has been associated with x-linked mental retardation.
Features and Benefits:
Antibody Bioguarantee
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Immunogen:
synthetic peptide corresponding to amino acids 40-59 of human cullin-4B, conjugated to KLH. The corresponding sequence is identical in rat and mouse cullin-4B and is not found in the homolog cullin-4A.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/97/2/157