Name :
Recombinant Human TIGIT Protein (His Tag), Amine-Labeled Biotinylated
Biological Activity :
Background :
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an “alternative self” mechanism for MHC class I inhibition. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Targets Immunotherapy Targeted Therapy
Biological Activity :
1.Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT His, Biotinylated (Cat:10917-H08H-B) at 2 μg/ml (100 μl/well) can bind Mouse CD155/PVR His & hFc (Cat:50259-M03H), the EC50 of Mouse CD155/PVR His & hFc is 50-250 ng/mL.2. Using the Octet RED System, the affinity constant (Kd) of TIGIT Protein, Human, Recombinant (His Tag), Biotinylated (Cat: 10917-H08H-B) bound to MITG7192A was 0.1 nM.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_776160.2
NCBI Gene ID :
Synonyms :
Synonyms :
T cell immunoreceptor with Ig and ITIM domains
Amino Acid Sequence :
Molecular Weight :
The recombinant TIGIT consists of 128 amino acids and predicts a molecular mass of 14.2 kDa.
Purity :
> 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human TIGIT (NP_776160.2) (Met1-Phe138) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Buffer Solution :
Lyophilized from sterile Sterile PBS.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
VSIG9 Protein, Human; VSTM3 Protein, Human; WUCAM Protein, Human TIGIT 背景信息 TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an “alternative self” mechanism for MHC class I inhibition. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Targets Immunotherapy Targeted Therapy
References & Citations :
Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. 36:40-45.Bechtel S., et al., (2007), The full-ORF clone resource of the German cDNA consortium.BMC Genomics 8:399-399.Yu X., Harden K., et al.,(2009), The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nat. Immunol. 10:48-57.
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