Name :
Recombinant Human USP30 Protein (SUMO Tag)
Biological Activity :
Background :
Ubiquitin specific peptidase 30, also known as USP30, is a deubiquitinating enzyme that is embedded in the mitochondrial outer membrane. Depletion of USP30 expression by RNA interference induced elongated and interconnected mitochondria, depending on the activities of the mitochondrial fusion factors mitofusins, without changing the expression levels of the key regulators for mitochondrial dynamics. Mitochondria were rescued from this abnormal phenotype by ectopic expression of USP30 in a manner dependent on its enzymatic activity. USP30 participates in the maintenance of mitochondrial morphology, a finding that provides new insight into the cellular function of deubiquitination.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
AAX36198.1
NCBI Gene ID :
Synonyms :
Synonyms :
ubiquitin specific peptidase 30
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human USP30 consists of 580 amino acids and predicts a molecular mass of 66.3 KDa. The apparent molecular mass of the protein is approximately 87 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity :
≥ 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human USP30 (AAX36198.1)(Thr48-Glu508) was fused with the SUMO tag at the N-terminus.
Buffer Solution :
Supplied as sterile 20mM Tris, 500mM NaCl, 20% Glycerol, 1mM TCEP, pH 8.0.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Wilkinson K. (1997) Regulation of ubiquitin-dependent processes by deubiquitinating enzymes. FASEB J. 11(14):1245-56. Ventii KH, et al. (2009) Activity and Cellular Roles of Ubiquitin-Specific Deubiquitinating Enzymes. Annual Review of Biochemistry. 78:363-97. Fang Y, et al. (2010) The potential role of ubiquitin c-terminal hydrolases in oncogenesis. Biochim Biophys Acta. 1806(1):1-6. (function(){if (!document.body) return;var js = “window[‘__CF$cv$params’]={r:’86b43bbc1afd880a’,t:’MTcxMTU5MzM5NC42OTIwMDA=’};_cpo=document.createElement(‘script’);_cpo.nonce=”,_cpo.src=’/cdn-cgi/challenge-platform/scripts/jsd/main.js’,document.getElementsByTagName(‘head’)[0].appendChild(_cpo);”;var _0xh = document.createElement(‘iframe’);_0xh.height = 1;_0xh.width = 1;_0xh.style.position = ‘absolute’;_0xh.style.top = 0;_0xh.style.left = 0;_0xh.style.border = ‘none’;_0xh.style.visibility = ‘hidden’;document.body.appendChild(_0xh);function handler() {var _0xi = _0xh.contentDocument || _0xh.contentWindow.document;if (_0xi) {var _0xj = _0xi.createElement(‘script’);_0xj.innerHTML = js;_0xi.getElementsByTagName(‘head’)[0].appendChild(_0xj);}}if (document.readyState !== ‘loading’) {handler();} else if (window.addEventListener) {document.addEventListener(‘DOMContentLoaded’, handler);} else {var prev = document.onreadystatechange || function () {};document.onreadystatechange = function (e) {prev(e);if (document.readyState !== ‘loading’) {document.onreadystatechange = prev;handler();}};}})();
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