Mplification efficiency and specificity (data not shown). Moreover, there had been reports that indicated that the Exiqon miRCURY assay, which also uses poly(A) tailing of the miRNAs, increased specificity utilizing LNA, but lead to a decrease in amplification efficiency [17,18]. To verify the amplification efficiency of the new assay, the amplification efficiencies of 10 miRNAs …
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The samples. The cDNAs were synthesized from the total RNAs for
The samples. The cDNAs were synthesized from the total RNAs for each sample with the Clontech SMARTerTM Ultra Low RNA kit. A sequencing library was prepared from each sample using an Illumina Paired End Sample Prep kit according to the manufacturer’s protocol. The average insert size of the libraries was 124 bp. Each cDNA library …
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Tor and LEM domain proteins such as emerin, MAN1 and Lap
Tor and LEM domain proteins such as emerin, MAN1 and Lap2. Membrane recruitment involves the nucleoplasmic domains of many of these INM proteins, which bind multiple chromatin-associated targets in vitro. For LBR, these potential interactors include DNA, histones and HP1. LEM domain proteins can interact with DNA but their DNA binding activity is not sufficient …
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Synthesis and fatty acid oxidation is not fully understood, as most
Synthesis and fatty acid oxidation is not fully understood, as most cells utilize regulatory mechanisms that prevent this inefficiency. One possibility is that the availability of fatty acids from the extracellular environment in vivo might differ depending on the tissues where they reside therefore Tm cells continuously store lipids that can be utilized for fatty …
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Hence, GALNT3 suppression could inhibit cell proliferation and invasion/ migration by
Hence, GALNT3 suppression could inhibit cell proliferation and invasion/ migration by destabilizing MUC1 and possibly other mucin-type glycoproteins and thus inducing the expression of cell adhesion proteins, although further in-depth analysis will be required to elucidate the precise mechanism of the GALNT3-MUC1 pathway in EOC cells. Our data specify some of the putative mechanisms of …
Itions, the strain was grown in chemically defined medium with or
Itions, the strain was grown in chemically defined medium with or without acetate supplementation (12 mM), under aerobic or anaerobic conditions. Analogous to what was observed with respect to the CO2 dependency, anaerobic growth of L. johnsonii NCC 533 depended more strictly on acetate supplementation as compared to aerobic growth, which could be sustained without …
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Were then passaged and frozen in DMEM containing 20 FBS and 10 dimethylsulfoxide
Were then passaged and frozen in DMEM containing 20 FBS and 10 dimethylsulfoxide for future use. Ear tissues were collected from a newborn piglet of the 18th generation in the No. 111-family and from an adult pig of the 23rd generation in the No. 133-family of Banna miniature inbred pig. The fibroblasts were isolated and …
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Ted in a further decrease of the wild-type residual transcript, from
Ted in a further decrease of the wild-type residual transcript, from about 5 to about 1 of the total FGG transcripts, thus confirming the role of this factor in promoting pseudoexon inclusion (194423-15-9 web Mirin web Figure 2B). In this case, to have a more accurate measure of the relative amount of the two splicing …
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An conventionally matured mDC. As shown in Figure 1, total MHC-Class I
An conventionally matured mDC. As shown in Figure 1, total MHC-Class I and HLA-DR molecules were 4-IBP cost up-regulated to a similar extent in DC matured with IRX-2 and conventional cytokines. Similar results were obtained when using DCs from HD (data not shown).IRX-2-matured DC Produce Higher Levels of IL-12p70 than Conventionally-matured DCIL-12p70 production by 25033180 …
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S, was used to assess the remodeling index (lesion diameter/reference
S, was used to assess the remodeling index (lesion diameter/reference diameter), which was considered positive when the diameter at the plaque site was 10 larger than that measured in the reference segment[7,11].2)256-slice CT Scanning TechniqueCT scans were performed using a 256-slice Brilliance iCT scanner (Philips Healthcare) that 4 IBP features a gantry rotation time of …
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