Focus Forming Unit (FFU) Reduction AssayFFU reduction assays were performed as previously described [14]. Approximately 200 FFU of virus were incubated with peptide in serum-free DMEM for 1 hr at room temperature before infecting LLC-MK2 cell monolayers for 1 hr at 37uC, and overlaying with media containing 0.85% (w/v) Sea-Plaque Agarose (Cambrex Bio Science, Rockland, …
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Results and Discussion Identification of WIKI as a Small Molecule Inhibitor of Wnt/?catenin SignalingTo make an assay for Wnt/?catenin signaling suitable for high throughput screening, we generated A375 melanoma cells stably infected with a ?catenin-activated luciferase reporter (BAR) [23,36] and selected populations in which luciferase activity is increased at least 4,000-fold by WNT3A. We tested …