However, the role of C/EBPs in IL-1b-mediated inflammatory responses in alveolar type II epithelial cells remains unknown

en in ” aB crystallin KO RPE exposed to the same concentration of H2O2. Results a-crystallin overexpressing RPE cells are resistant to oxidative stress induced cell death We generated a-crystallin overexpressing stable cell lines and demonstrated that aA crystallin or aB crystallin overexpressing cells were more resistant to H2O2-induced cell death than vector control cells. Overexpression of aA crystallin or aB crystallin resulted in 10% cell death at concentrations of H2O2 that caused 30% cell death in control cells. Further, caspase 3 activation was inhibited in acrystallin overexpressing cells exposed to H2O2. The dose and duration of H2O2 used in these studies were 150 mM and 24 h, respectively, as has been validated in our previous work. Higher thiol levels provide protection from oxidative stress in a-crystallin overexpressing cells We next investigated the link between a-crystallin expression, intracellular thiol levels and enhanced cell survival in oxidative stress. Our data revealed a significant 2-fold increase in cellular GSH levels in a-crystallin overexpressing clones when compared to controls. One of the main mechanisms for elevation of cellular GSH is increased biosynthesis catalyzed by the rate-limiting enzyme glutamate-cysteine ligase . The increase in total GSH levels was associated with significant upregulation of the gene and protein expression of the catalytic unit of GCL but not GCLM, the modifier unit of GCL. Mitochondrial fractions from a-crystallin overexpressing cells had significantly higher GSH levels after treatment with 150 mM H2O2 for 24 h. The Aphrodine chemical information magnitude of increase in GSH level in cytosol, MRP-related GSH transporters in RPE 17622149” cells We then proceeded to characterize the transporter mediating GSH efflux from RPE cells. Several MRPs are known to mediate GSH efflux in mammalian cells. To determine the presence of MRPs in RPE, MRP mRNA levels were analyzed by RT-PCR. RNA isolated from RPE cells was amplified using specific MRP primer sequences. mRNAs encoding for MRP1, MRP2, MRP3, MRP4, MRP5, MRP6, and MRP7 were detected in RPE cells. MRP1 was the most abundant of the MRP family members in RPE. All further experiments were performed with MRP1 because it is the most well characterized MRP with respect to efflux of GSH and GSSG. MRP1-Mediated GSH Efflux in RPE Cells Localization of MRP1 in a-Crystallin overexpressing RPE cells In subconfluent ARPE cells, MRP1 is predominantly localized in the plasma membrane and the staining pattern is punctate. In human polarized RPE monolayers, we observed lateral membrane localization of MRP1. Biotinylation of intact cells with subsequent immunoblot analysis revealed surface localization of MRP1 in the membrane fraction. These studies further established that membrane expression of MRP1 was almost three fold higher in aB crystallin overexpressing cells than vector control cells which correlated well with the increased GSH efflux in a-crystallin overexpressing cells. In addition, cellular MRP1 expression showed a.2.5 fold increase in aB crystallin overexpressing cells as compared to vector control cells. Furthermore, consistent with GSH efflux under oxidative stress, we observed a.2 fold increase in MRP1 expression only in vector control cells subjected to oxidative stress. Having established that increased a-crystallin levels increased MRP1 expression, we then investigated whether knocking down of aB crystallin could affect the expression of MRP1. As seen in Fig. 5E, a signific

212 thoughts on “However, the role of C/EBPs in IL-1b-mediated inflammatory responses in alveolar type II epithelial cells remains unknown”

  1. Habe die Aufregung um das Projekt immer leicht irritiert beobachtet, aus der Ferne und mit grünem Planer – Hintergrund. Mir gefiel bisher, was ich sah – waren bisher nur die Pläne, Fotos und der Zustand am Jahresanfang. Würde mich insnretsieree, ob man sich auch als Protestierer mit dem Ergebnis anfreunden kann. Wie ist denn die Stimmung jetzt?

  2. ist nicht juhu so ein tolles programm?was mich bei jaiku nervt: mein text, den ich oben ins eingabefeld tippe, erscheint nur unglaublich langsam buchstabe für buchstabe … und wenn ich während dem warten in ein anderes fenster klicke, vergisst es den rest vom text :( ich hab keine ahnung, woran das liegt.

  3. I'm looking forward to blogging here. I've always got a ton of stuff to say. Today, was mostly spent writing descriptions of videos. Tomorrow I hope to test one and then load it. Speaking of loading up,….I did succeed in uploading Sherridan Smith's discussion about his Celtic bull sculptures, both on YouTube and on Facebook…..But I'm having a heck of a time, looking for mama

  4. The kick has me cracking up. Language development is indeed interesting (and hilarious!) I’ve done the bouncy houses and Chuck E Cheese for other people’s kids’ parties but not my own. And my reasons are proven every time I return.Arnebya’s last awesome post…

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  6. I agree. People in general, and kids in particular, have well-honed bullshit detectors these days. Evangelizing would be seen (rightly) as just another marketing campaign.Also, the dominance of evangelical Christianity and that very Calvinist version of the faith that they promote fits too well with the supremacist ideology that dominates Republican politics these days.

  7. hi Bhargavaall the best in ur efforts.networking is a loosely used word. every recruitment is a give-take process. if an interviewee/candidate has promise, and be able to get the opening for an interview thru other means, then networking is not required too. but outside of campus, it is not easy to get referred, unless u know people. cheers

  8. nie byłoby problemu , bo nie byloby Wałęsy ,gdyby pan i pozostali co znali jego przeszłość wykazali nieco determinacji w pamiętnych czasach, nam na Śląsku jakoś udało się zdemaskować konfidenta!!!Ciekawi mnie tylko czy pani Danuta nadal nie straciła wiary w szczęście męza w grach liczbowych .

  9. great write-up…excellent publish, really educational. I ponder the main reason the opposite specialists when using the area won’t really area these types of. You truly must move ahead types very personal crafting. I is going to be just confident, you might have a ex…

  10. ich weiß nicht, wie burberry das geschafft hat, aber dieser gürtel macht mich zum lemming! der ist nicht mal schön. aber ich bilde mir ein dass ich ihn haben muss.

  11. What kind of soup are you having?I owuld do a pumpkin/squash soup then serve it in th epumpkin, that would also fit your theme, and squash are cheap. Buy them this summer at the farmers market, they are WAY cheap then and you can keep then for months at a time.Or uy them at the farmers market, pre cook a bit and freeze.

  12. disse:Prezados o item 7 reque um pouco atenção ou cuidado, pois devido ao tipo de terra que tiver no vaso a água pode escorrer toda no primeiro dia, havendo necessidade de uma “tampa furada” para reduzir e adequar a vazão.

  13. I’m not sure the Lord will respond to the curious. Now, he may be more than curious (experiment), I don’t know for sure.I can see a “reality” TV show coming out where they attempt to contact God or the devil using various means and mediums.

  14. I can't believe she's splitting up the pair! We have two brothers and they would be LOST without each other!!!!But congrats on getting one of them….but don't be surprised if she calls to offer the other one later because of how he's reacting to being split up!

  15. Il fallait que je te le dise. ça me fait du mal, tu dois me savoir.Habituellement, je me serais retenu, le genre de retenue humble et super amicale.Parce que ma bonne éducation fait que je ne dois pas tirer sur l’ambulance.Mais là, point n’en faut plus.J’ai craqué.Voilà:« ha ha ha ! branquignole ! ah le gars ! pouah ! »Je n’aide jamais les gens qui bossent sur Mac.PS: par contre, t’aurais un petit Adobe Premiere de derrière les fagots, je ne dis pas

  16. fordert von FDP ein Einlenken beim Mindestlohn Emotionen: 22* In Blogs gefunden: Podolski zu Arsenal London Oder zu Schalke Oder bleibt er dochDer Express schreibt dass Podolski noch gar nichts unterschrieben habe sondern höchstens mit

  17. É sempre uma busca. Vivemos por ela. Talvez valha-nos o exercício de buscarmos a calma do estio dentro de nós mesmos, sem marido, filhos, amiga, doritos ou outros vícios. E depois de achada a quietude, aí sim, olhar adiante e começar a receber de novo, de quem estiver pronto e disposto a dar.É um texto bonito, e reflexivo.

  18. Luv these cards, and the GOLD outlining!!!! Isn’t it interesting to see how a simple, beautiful stamp or saying can become an elegant, gorgeous card. Thank you for sharing. I get so inspired , , , , (that’s my dust heading off to the craft room!!!

  19. Can I simply say what a relief to find somebody who really knows what theyre speaking about on the internet. You positively know how to bring a difficulty to light and make it important. More individuals have to read this and understand this facet of the story. I cant imagine youre no more standard because you positively have the gift.

  20. Love is far from the perfect teammate. At UCLA he consistently complained about not getting enough touches; in Minnesota he gave up on the team at the end of last season. He’s far more selfish than most realize but he gets a pass because of cliches about white, unathletic players.

  21. Hola Marc:El proyecto pinta muy bien, pero registré a Gabriel Arango como marca y tuve un par de problemas/incidencias para las que puse un ticket y no he recibido ninguna respuesta.¿Hay alguien atendiendo el soporte a usuarios?Vota el comentario: 0  1

  22. Non hanno mai fatto troppo peso sul “ma noi ti paghiamo qui, ti paghiamo di là”, perchè hanno sempre sostenuto che è giusto che paghino loro le mie spese, però sì, abbandonando il lavoro tornerei a sentirmi decisamente meno libero

  23. My heart goes out to you, miscarriage is a very difficult thing to go through. {hugs} and lots of prayers coming your way that you are at peace today as you remember Max.xoxo C

  24. Hello, I need a catalook expert to help finish the final few skin issues on the checkout / basket pagePlease can someone who has worked with this module get in touch with me?Thanks

  25. Escribe tu comentario Puedes usar las siguientes etiquetas HTML: <a> <abbr> <acronym> <b> <blockquote> <cite> <code> <del> <em> <i> <q> <strike> <strong> var RecaptchaOptions = { theme : ‘red’, lang : ‘en’ , tabindex : 5 };   #submit {display:none;}

  26. The UK IPO also has a substantial operating surplus of income over expenditure. Rather than returning this to the IPO's "customers" in the form of reduced fees, or giving it to the staff as a productivity bonus [increments have been frozen for the past 3 years], the BIS is proposing to appropriate the surplus itself. The basis is that the IPO is effectively a company in which the BIS is the sole share holder, and so the surplus can be paid to it as a dividend on its share holding.

  27. That tosh colorway is spectacular! I’ve been seeing a lot of Tosh today in the “love” category, and I completely understand why.As for the Berroco – they do an amazingly good job with most all of their yarns, even acrylic blends. And with the pattern support, it’s definitely great yarn value. Besides, how can you not like a yarn company with Norah Gaughan AND Cirilia Rose on the payroll?

  28. to be a contender for the new James Bond franchise a while ago! He does have hot Venus in cool virgo, and the moon saturn conjunction is very Saturnian indeed. I don't know his Asc, but its definitely not an overly expressive one. lol He also has hot Mars in cool Virgo, and I guess that warm Leo Moon gets all squashed by being conjunct Saturn. I was sort of wondering why he was becoming a sex symbol, but now its obvious. He's got that 'detached' hotness going on!!

  29. danielle: awesome sentiment – thanks for sharing!korken: thanks so much for the support & enthusiasm dude – really appreciate it. i look forward to hearing about your upcoming adventures, too! :-)

  30. to a friend, “this winter feels a lot warmer than past winters…” — then I realized I just FINALLY got a warm coat and boots!Let’s go get cocoa or cider sometime and sport our winter gear! Or, you can test how your coat holds up to the elements and trek over to my new place!

  31. Hej, okno w Niebie jest znów otwarte.Kto chce i w to wierzy, może przejrzeć się w jego szybach.Nie pozwól, by słabsi od Ciebie budzili w TOBIE negatywne emocje. To tak, jakby wygrali.A przecież, sięgając po pieniądze najsłabszych, sami przegrywają, tracąc resztki człowieczeństwa.Pozdrawiam Cię!

  32. So much of being successful is just a state of mind! I feel insanely, wildly successful. Beyond my biggest dreams actually. Yet, but some people’s standards, I’m sure I haven’t scratched the surface of what “successful” means to them.And isn’t it funny how getting into shape and getting finances in order always go hand in hand?!?

  33. Caren – his grandfather was obsessed with squares. He was a architect and when he hit forty, he disappeared for a week without telling anyone and then returned and announced to his wife that he was going to become an artist. This is just a small piece of what he created – he was quite prolific and we have tons of his work.

  34. er ening i at løsningene ofte løses i sentrum, eller sagt med andre ord. løsningene er ett resultat av underklassen(venstresiden) og overklassen(høyresiden) sine interressekamper. tese, antitese = syntese = venstre:P

  35. i graduated in Bsc in genetic and now study in Msc can i apply forr you scholarship undergraduated and expect to accept me . my bachelor univesity is one of the best in iran .

  36. พูดว่า:When I initially commented I clicked the Notify me when new comments are added checkbox and now each and every time a comment is added I get 4 emails with the same comment.

  37. I was relieved to find that not all of these were primates. The Phoca Jubata looks like it belongs on a heraldic crest from another planet. As for the bats, well, they should meet up with those to be found in the National Museum here. Though I’m not sure whether it’s wise to encourage multi-bat-species orgies…

  38. Video is a good way to explain anything. Thanks for sharing it. Vitamin B17 is great for health. It is found in many foods. Vitamin B17 face pack brings shine to skin, soothes troubled skin, relieves sun burns and many other benefits.

  39. SUGGESTION:1. From what I’ve heard on TV, LPI is a rival for PSSI, if I’m not mistaken. 2. Our national team is in its victorious moment …3. …I think politicians out there must not intervene/interfere our football team affairs. 4. I think they shouldn’t take advantage from the rising football team for their own benefits.

  40. The best phishing site is found on Igoogle as a gadget. Spotcrime is a website that provides crime info for your neighborhood. No problem except it requires your email address and password: Get FREE Local Crime AlertsStay updated with inbox alerts for your neighborhood!Email:Password:Address:e.g. 100 Main Str, Baltimore, MDPlease include Street and City,State or Zip code

  41. excellent work. Sequal Pro is my favorite database mgmt tool, but I always wanted to make a sort of custom content manager for web clients and that sort. test seems to work the real debate, will apple every create an official mysql framework for objective-c? or will we have to use sqlite3 or mess around with webkit?thanks for sharing!

  42. 7  22Der Röhrenfernseher ist doch nur Tarnung, wenn die Tante vom Sozialamt zur Kontrolle kommt. Hast du nicht den schicken Flachbildschirm darüber gesehen, der verschwindet dann natürlich im Schlafzimmerschrank.Mit seinen Tanzauftritten, verdient der Kerl doch Millionen und kassiert nebenbei auch noch Sozialhilfe.

  43. Kraman dit :Il faut cependant tenir en compte un truc, le gouvernement prend un peu la responsabilité de ses actes. Quand il a interdit les commandites des compagnies de tabac, il aurait condamné plusieurs festivaux s’ils n’avait pas pris la relève que ce soit directement ou par le billet des sociétés d’État(principalement la SAQ, Loto-Québec et Hydro-Québec). Bon maintenant, est-ce que c’était une bonne idée d’interdire ces commandites, personnellement, je crois que non, mais c’est un autre débat.

  44. Du vécu ? c’est tout à fait ça.Et tout à fait d’accord avec vous pour enseigner dès le CM2 l’utilisation intelligente et les dangers potentiels de liens anodins sur les moteurs et pages web. Mais avec quels moyens ? Des instits formés comme pour les cours d’anglais ?Attention, Monsieur Vidberg, dès qu’il est question de bouc, Lucifer n’est pas loin.

  45. I'm not making waffles (though I'm now craving them), but I agree with Scott – I'm thrilled to have such a nice shout-out from you! I've been following you on Facebook for 100 years, but I'm excited to hear your BlogVoice, too. :)

  46. Wonderful work! That is the kind of info that should beshared around the internet. Disgrace on the search engines fornow not positioning this post upper! Come on over anddiscuss with my site . Thank you =)

  47. Cicci skriver:Hej! Kommer inte riktigt ihåg hur jag hittade till dig men ett är säkert, när jag hittade dig blev du en favorit som jag besöker dagligen sedan dess!

  48. Ola, estou com problema para instalar o AutoCad 2012. Eu baixei e descompactei ele com o o programa HJ-SPLIT e quando vou vou instalar ele pelo Power ISO ele da a seguinte mensagem:Erro nos dados (Verificação cíclica de redundancia)C:\\users\\\\appdata\\local\\temp\\_ai565b.tmp\\x86\\pt-br\\components\\adr2012\\setupdesignreview2012.msiAguardo retorno.alina Responde:olá, estou com o mesmo problema na instalação! vc conseguiu resolver??

  49. ArchiTegn, der er Arkitektskolens butik her i Aarhus, har rigtig mange forskellige til 20 kr. rullen. Har ogsÃ¥ set dem i BoxDeLux i Graven til 25 kr. Skal have købt nogle ruller selv, men gÃ¥r altid i baglÃ¥s nÃ¥r jeg stÃ¥r foran dem, for vil jo helst have dem alle! Din gang ser forresten super fed ud. MVH Pernille –

  50. jun29Fede Buenas a todos! Con todo respeto, eh, ¿por qué no se van a vivir a EEUU? Si ahí pueden comprar televisores baratos y vivir una vida capitalista a pleno, gozando de todos los beneficios que tienen ellos…

  51. Tenho um desejo enorme de fazer o Curso de Biblioteconomia. Agora surgindo esta oportunidade de fazê-lo à distância, sinto que tenho nova chance. Gostaria de obter todas as informações. Moro em Araguaína/TO, se essa região será contemplada com o cuso à distância.

  52. Muistan sinun aikaisemmin kertoneen ensimmäisestä lapsestasi, ja en voi mitenkään unohtaa sitä. Nyt tämä kaunis pitkä kirjoitus avasi vielä enemmän. Ajattelen kanssasi monesta asiasta samalla tavalla, vaikka en ole mitään vastaavaa kokenutkaan. Eikä meilläkään kutsuta lapsia enkeleiksi, sillä lähipiirissä on enkelilapsia.

  53. This really is my first-time i check out here. I identified a multitude of entertaining stuff as part of your blog, particularly its discourse. From the tons of comments on the posts, I guess I’m not the only one having most of the enjoyment the following! Maintain within the excellent job.

  54. I know how you feel! My husband and I decided not to buy big gifts for Christmas after stressing for a week over what to get, whether we could justify the shipping on the heavy item we really wanted to get DD, and so on. We’re just doing a handmade pair of pajamas each (I finished each in a day) and a book and DVD each we’d bought earlier in the year. My days have been so much freer to simply enjoy my family!!!

  55. Gracias Isidra, lo veo mucho en mis sesiones de pareja. Cuando hay un acto de infidelidad lo primero que sucede es el juego de culpas lo cual no ayuda al proceso de sanación, por el contrario, profundiza las heridas. Abrazos

  56. Just want to say your article is as astonishing. The clearness in your post is just nice and i could suppose you are knowledgeable on this subject. Fine together with your permission allow me to snatch your feed to stay up to date with approaching post. Thanks a million and please carry on the gratifying work.

  57. Bonjour,Oui toutes les options de calcul sont bien activéeset fonctionnent pour d’autres fonctionsje saisi cette occasion de presenter mes remerciementsparce que j’ai appris beaucoup de choses à travers vos cours precieux aussi bien sur ce blog que sur polykromie et sur les lettresles videos …

  58. Please give me a choice to remove bounced mails from my lists. I get a lot of bounced mails which gets accumulated and choke up my bandwidth and waste a lot of storage.

  59. Seriously?Vaccines have existed since the 1700s. They’ve been used as rouitne childhood vaccinations in industrialized countries since the 1950s.World Population (approx)1800- 1 billion1960- 3 billion2011- 7 billionIf vaccines are an attempt to reduce world population, it’s obviously a huge failure.Also, please go look up the definition of eugenics and come back and explain how that applies to something given to every race, sex, age, religion, etc

  60. Kako!!!, si en casa no te acompañan en estos sabores yo estoy dispuesta a coger el avión direccion Zürich a compartir!!!…me parece realmente original tu receta y estoy segura de que Laube estará encantada!!Y yo dando vueltas al tema y no me inspiro…me estoy poniendo nerviosa!!!!!, al final no sé si me presentaré!!!Bueno amiga, feliz fin de veras que me encantó tu receta!!!!Besitos!!!

  61. Aussies have 4 priorities:1. Their Mates2. Footie (sports in general)3. Beer4. Their dogThat leaves Australian women very amenable to the attentions of Southern Gentlemen. God I miss that country.

  62. “I would agree with Jamie that it’s probably about statistics and about which demographic groups are more likely to travel.”Personally, I wouldn’t agree with ‘nice’ Mr Stokes’ theory.I actually looked up the statistics – immigration from A8 EU nations (within which Poles form the majority) after EU accession showed a disparity of 56:44 in favour of men.Historically it has nearly always been men rather than women who have chosen to migrate.

  63. This is such an interesting combo, Mary! Who'd ever think of avocados and curry powder in the same soup? Not me, at least 😉 This is definitely worth a try!

  64. You look very different in the last pictures just by the fact that you use lipstick, it looks good (:& on the first pictures, I LOOOOVE how looks your hair, sooo black. LOVE IT.Well. See ya(:

  65. Third Flower…My wife and that i are already now delighted that Albert could carry out his research on account of the concepts he had via your web page. It truly is on occasion perplexing to simply normally be giving freely actions which many people might have been …

  66. how many unique visits you are getting,…how much time a visitor spends on your site, how targeted is your traffic, etc. – everything is to be considered.some blogs may do well with pay-per-click (ppc) ads like google adsense while others may earn better with cost per acquisition…

  67. Learning about hunger is essential for every  citizen including school age children.  They should know it’s not just across the world but in our own communities.  Please provide breakdowns by town,city and county and what small thing each citizen can do to help.

  68. Thanks a bunch for sharing this with all folks you really know what you’re talking about! Bookmarked. Please also talk over with my web site =). We could have a link exchange agreement between us!

  69. HOLY. MOLY. I mean, it makes total sense that there would still be bombs around, but I had never thought about it. Soooo that's a benefit of being on the internet so we Americans can get a better perspective on the world. Anyway, I'm glad that everyone is okay. And there isn't really a better episode as an aftermath to that, is there?

  70. I don’t know whether it’s just me or if everyone else experiencing issues with your blog. It appears as if some of the written text within your content are running off the screen. Can someone else please comment and let me know if this is happening to them as well? This could be a issue with my internet browser because I’ve had this happen before. Thanks

  71. Hallo ihr,mir gefällt der 679 Pennies For Heaven am besten, da ich schon lange auf der Suche nach einem goldenen Lack bin und bisher nicht den passenden gefunden habe :-)Liebe Grüße

  72. hmmmm, after reading about the Malibu house story, I was thinking……how would this whole recession/bank failure/real estate bubble thing have gone down MINUS the copious amounts of cocaine?

  73. hello. i liked a lot of your video and in some point what you say of everything is a remix can be taken as true, but, when you use the words in a dictionary and you make a book, you inspired yourself in the words that exist to tell a story that is something new, just put in a new order. As i said, i liked the videos, but i would like for you to consider the making of a video with what is not a remix. thank for your attention and kip up with the good work.

  74. bonjour a tousvoila il y a 1 mois j ai eu mon permis suspendu pour une duree de 4 mois (alcoolemie) il est clair que j ai un probleme avec l alcool.Je n ai pas parle de cette situation a personne .je suis passee au tribunal vendredi et ma sanction a ete reduite a deux n ai pas bu depuis mon arrestation.pensez vous que je puisse recupere mon permis ?j ai rendez vous avec l additologue dans 15 c vrai on se sent mieux sans alcool . merci pour vos temoignages et vos reponses.

  75. To bad it’s over I hope you are posting workouts back soon! To bad my forearm got injuried, think I trained to hard by watching your vids, its your folt kevin :p I think my tendons aren’t ready for it yet …Can’t train for 2 or 3 weeks getting insane!Good luck in the future.

  76. Hey – nice weblog, simply looking round some blogs, seems a fairly good platform You Are using. I’m at the moment using Drupal for a number of of my sites but looking to change certainly one of them over to a platform very much the identical to yours as a trial run. Anything in particular you’ll advocate about it?

  77. Hello there, just became aware of your blog through Google, and found that it’s really informative. I am gonna watch out for brussels. I’ll appreciate if you continue this in future. Numerous people will be benefited from your writing. Cheers!

  78. Ahh…..Rumpole… beautiful memories of the south island, Wanaka and skiing for the month of July in delightful NZ. Blessed be the kiwi. . Lovely place you’ve got there!!! Miss it!

  79. Ma avete visto anche voi il 'meteorite' cadere al minuto 0:46 (ripresa dall'interno) ??? E' vero, a 0.47 passa qualcosa. Ma scorrendo il filmato fotogramma per fotogramma l'"oggetto" non sembra avere una traiettoria continua: in particolare, sembra scomparire di colpo prima di finire dietro al casco. Boh.

  80. If you’ve made it this far, then check out the following article when you have time. It continues to head to greener pastures. Enjoy and God be with you, always: GO DEEP!!!

  81. Thanks a bunch for sharing this with all of us you really know what you are talking about! Bookmarked. Please also visit my site =). We could have a link exchange contract between us!

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