A 200 ml of thiobarbituric acid reagent was added to 100 ml of the sperm suspension

Glu interferes with the expression of the astrocyte transporter subtypes, excitatory amino acid transporter 1/glutamate/aspartate transporter and EAAT2/glutamate transporter-1 . To explore the effects of Glu on the expression of Glu transporter genes in cultured Aglafoline site astrocytes from wild-type and MeCP2-null mouse brains, we asked whether treatment with 1.0 mM Glu altered expression of EAAT1 and EAAT2 mRNA, using a semi-quantitative RTPCR assay. EAAT1 and EAAT2 mRNA were expressed in both wild-type and MeCP2-null astrocytes, and were slightly higher in controls than in MeCP2-null astrocytes. Both EAAT1 and EAAT2 mRNA levels were altered in the control astrocytes after treatment with 1.0 mM Glu. EAAT1 mRNA levels decreased significantly in the wild-type astrocytes, both 12 h and 24 h after treatment with Glu. In contrast, EAAT1 decreased significantly in the MeCP2-null astrocytes, at 12 h but not 24 h after treatment. As with EAAT1, EAAT2 mRNA levels also decreased significantly in the control astrocytes, both 12 h and 24 h after treatment. However, EAAT2 decreased significantly in MeCP2-null astrocytes, 24 h but not 12 h after treatment. In addition, the effects of Glu on EAAT1 and EAAT2 relative fold expression at 12 h were altered in the MeCP2-null astrocytes. These 20688974 results suggest that the loss of MeCP2 leads to transcriptional dysregulation of these genes, either directly or indirectly. One important enzyme that plays a role in the Glu metabolic pathway is glutamine synthetase . GS is mainly located in astrocytes; cultured astrocytes response to Glu with increased GS expression. Consistent with this, 1.0 mM Glu treatment stimulated GS mRNA expression in both the wildtype and MeCP2-null astrocytes about 1.2-fold after 12 h but not 24 h. In addition, MeCP2 deficiency did not modify the Results Characterization of MeCP2-null astrocytes It was recently reported that MeCP2 is normally present not only in neurons but also in glia, including astrocytes, oligodenrocytes, and microglia. To determine the roles of MeCP2 in astrocytes, we cultured cerebral cortex astrocytes from both wild-type and MeCP2-null mouse brains. MeCP2-null astrocytes exhibited a large, flattened, polygonal shape identical to that of the wild-type astrocytes, suggesting that normal patterns of cellular recognition and contact were present. Semi-quantitative RT-PCR using primer sets that specifically amplify two splice variants, Mecp2 e1 and e2, showed that control astrocytes expressed Mecp2 e1 and e2, whereas neither Mecp2 variant was detectable in MeCP2-null astrocytes. We further confirmed expression of MeCP2 by immunocytochemical staining of astrocytes. In control samples, almost all GFAP-positive cells exhibited clear nuclear MeCP2 immunoreactivity in astrocytes, but no immunoreactivity was observed in MeCP2-null astrocytes. MeCP2 has been reported to be involved in regulation of astroglial gene expression. Consistent with this, GFAP levels were significantly higher in MeCP2-null astrocytes. Similarly, the expression of S100b, ” another astrocyte maturation marker, was significantly upregulated by MeCP2 deficiency. These results show that MeCP2 deficiency upregulates astroglial gene expression in astrocytes. To compare the growth of the wild-type and MeCP2-null astrocytes, we counted total cell number at each passage. As passage number increased, the cell growth rate decreased Characterization of MeCP2-Deficient Astrocytes effects of Glu on GS mRNA relative fold expression

267 thoughts on “A 200 ml of thiobarbituric acid reagent was added to 100 ml of the sperm suspension”

  1. Jonas e onde é que foi dito que existiam 100 milhões para gastar em reforços ou que esse valor seria o orçamento da próxima época?Olhe 30 já foram gastos só para cumprir com coosrmmispos anteriores.

  2. Sorry I can’t help with your actual Q, but:No matter what, you have to answer truthfully – and if you’re going to be eligible for it you will, if not you won’t. No sense in worrying about it, is there?I realize something like this you can’t help but to worry about – but I meant don’t INDULGE yourself in your worrying. TRY not to think about it, if any questions or thoughts come up like this immediately get up and go do something else, etc.Best of luck, with both easing the worrying and qualifying again!

  3. Es que el error que cometen los admiradores de esta cinta es pensar que sus detractores prefieren “tiros y efectos especiales”.No tiene porque ser así. A mi me encantan las viejas películas del Oeste estilo Clint Eastwood.Que a uno no le haya gustado esta película no quiere decir que le guste Monstruoso, Rambo IV u otras basuras.La pelicula estará muy bien hecha pero el guión me parece malísimo. Desde luego no pienso leerme la novela.

  4. Fortunately, this is a big thing in the Danish press. Here’s an editorial from .Related, our minister of culture, Brian Mikkelsen, has a great OpEd here: . It is written on the occasion of Trykkefrihedsselskabet awarding Kurt Westergaard (of Motoon fame) a prize for his courage.Further, we’re badding those cursed headscarves from our courtrooms: (this article is in English).Looks like we Danes are doing well, again :)

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  6. THIS BOOK IS ONE I LIVE BY AS A CHILD OVER COMING THINGS TWAS READ TO ME AN I READ IT TO MY CHILDREN AND HOPE TO READ IT TO MY FUTURE GRAND CHILDREN AND EXPLAIN WHAT IT HAS DONE AND IS DOING FOR ME. IT RECENTLY (IN THE LAST 20 YEARS) kept me going an helped me recover from alot.

  7. Water conversation is going to be a major issue in the near future. The government is predicting 46 states will face water shortages over the next 5 years. Toilets waste more water than any other appliance in the home. Low flow and dual flush toilets are great products for conserving water.

  8. bojamalFirst of all I don’t care neither about your feud with NBK or NBK’s services But the add on the stupid car is not JUST an advertising scheme. The car is donated by NBK to medicate the elderly at home.

  9. Also, MM, this forum needs moderation. There is no benefit to permitting the use of empty derogatory terms like "faggot" and "asshole." Why not follow the example of LA and sanitize the language so that it is not unnecessarily offensive?Since the whole faggot and asshole thing came from the original post, it would be a tad hypocritical for him to censor those terms in the comments section.

  10. This is a great post James, thanks. I laughed about your first blog posts – mine were musings about the importance of confidence in communities. it is sort of related to our business but honestly, who did I think I was!You are so right about none of this being a quick win for business – and it needs such long term commitment.We are reminding ourselves with some of your tips – thanks!

  11. La mia nonna diceva:" Chi ha buon naso, ha buon tappo." E con tappo indicava quello che pensate, così tutte le volte che vedo un uomo con un naso inguardabile, penso subito che magari la moglie se lo è scelta x quel motivo lì.Vabbè.

  12. Have you ever considered creating an ebook or guest authoring on other blogs? I have a blog centered on the same ideas you discuss and would really like to have you share some stories/information. I know my subscribers would value your work. If you are even remotely interested, feel free to shoot me an email.

  13. Menudo despiste.Ahora resulta que lo de este oscuro personaje (que es dado a simultanear militancia ,,,pero desde puestos de dirección, of course, como hizo con EA y el grupúsculo POR-Redes) o lo de R. Aguilar, P.A Rios de asesor de Rubalcaba es de lo más natural…???? Así nos va.

  14. I like this part: “Now Trump is demanding that Obama reveal his college grades, so we can see just how much his career has been boosted by racial preference and other favors.”As Trump has told us, he’s friendly with “the blacks,” so he surely knows that they can’t make it on their own. Trump won’t run for president. He’s a con artist promoting himself.Why does the search for a candidate continue? If any of the Republican candidates can defeat Obama in an election, just pick one and get on with it.Time’s a wasting.

  15. Ich war von Modern Warfare 3 so enttäuscht dass ich auch gesagt hatte “Das ist’s mit CoD gewesen, bis hierhin und nicht weiter!”, aber nachdem ich den Multiplayer auf der gamescom angespielt und die ganzen Trailer gesehen hatte, musst ich CoD doch noch ne Chance geben Sieht einfach nur cool aus und der Multiplayer Part war auch mal wieder zu gebrauchen ^^

  16. I love this series so much. If this blog post alone can get tears trying to form then reading the end of the story is going to have me pouring my eyes out… that or I am really emotional and need chocolate. I’m not sure. I think it shall be both.I want to re-read the entire series. I never reviewed them as I read them one by one and I am regretting that. It’s something I want to fix.

  17. Men Ã¥h! Enda tanken när jag läser detta: herregud, finns det dinosaurier (genusblinda) mitt ibland oss fortfarande …Heja dig, och sluta för fan aldrig med det du gör.

  18. Overall Rating Customer Service Ease of Use Accuracy Value For Money I used Intelius because of the reviews on this site and their percent of accuracy in performing a reverse lookup. The report took 24 hours before it was ready for viewing. Unfortunately, the report had the wrong city by 50 miles, no address, and no last name. Since I already knew the person’s address and first name, the report was useless to me.

  19. Granny, I got so wrapped up in the issue at hand last night that I failed to notice your posting. I’m sorry to hear about the unhappy circumstances, and I wish you the best, and I will look forward to your return here when you are up to it.

  20. Cam liked being swaddled at first, then hated it. Now for about the past week when she gets super fussy at night, I’ve been using the sleep sack things. She is getting used to them again now, but I’m not sure how long that will last. We don’t swaddle her hands though as she loves having them free. I’d say about half the time she isn’t swaddled though.

  21. yan on hai..nk tnya pada yg pakar…aku nk oc aku nyer proc tp x sure..agak2 bape leh up ngan proc aku ni..tq…ni spec pc aku stakat niamd athlon 64 x2 dual core 2.50ghzram ddr2 800mhz 2gb/4slotmobo abit nf-m2pvgc forsa 8600gt 512mbpsu 5oowatt

  22. , we have to stay abreast of what they’re saying and thinking, as their policies are based on them. The fact that they belittle the people shows what they think of us, and, in the end, what they’ll do to us.

  23. Dissemination…I am very happy to read this. This is the kind of manual that needs to be given and not the accidental misinformation that is at the other blogs. Appreciate your sharing this best doc….

  24. Thanks Peggy! I always love hearing from you and am glad that you took a minute to leave a note on here. And yep! Still just living like i do. You didn’t think i’d let a little cancer slow me down did you!??Haha. I hope everything is going well with you in Atlanta, and we’ll have to have one of our infamous wine nights when I get back in town!Stay in touch!!!LoveRob

  25. Hi there, I have just come across your blog and it’s just lovely. I’ve booked marked your website also ready for Christmas.I will be coming back to read more.Nice to read you xCathrine x

  26. No Black Swan?I think that it could easily replace Youth in Revolt (which I loved the hell out of the first half and found the rest to be plodding), The Social Network (I didn’t get the love for this at all – I found it to be an uninteresting story about an unlikeable person) or Kick Ass (which I found just okay – I thought Scott Pilgrim was the movie Kick Ass was trying to be).But hey, that’s just me.

  27. é só vc apertar esc e vai no primeira pergunta escrita spiel speicher alguma coisa assim dai click ali e espera ate sumir umas letras q ira aparecer em cima e saia

  28. ach so, jadi ini toh kopi aroma. namanya dulu sering dengar waktu masih tinggal di Bandung. konon terkenal. dibanding kopi luwak, enak mana Ga?Yoga:Kopi Luwak nggak cocok buat yang lambungnya sensitif, sangat asam. Kalau ditanya enak mana, susah aku jawabnya Li, karena aku demen dua-duanya.

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  30. Great ShowBut I tink Alan Greenspan would have a Heart Attack if Peter Schiff & Max went after himAnd tears no way he’d agree cause his secratry would Google ya guys and get to know what ya guys are all aboutAlan Greenspan Probably uses the DVD tray on a comp. as a Coffee Cup holderlolololololROFLHic

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  33. That’s really cool that you are taking your wife to Lameau Field for her first time, Dave. I expect the Packers to beat da Bears whether it is a close game or a blowout. Let’s see the Pack move on to the playoffs & make some noise. Go Pack!

  34. BOYCOTT AMERICAN WOMEN! http://www.BoycottBitches.com I am an American man, and I have decided to boycott American women. In a nutshell, American women are the most likely to cheat on you, to divorce you, to get fat, to steal half of your money in the divorce courts, don’t know how to cook or clean, don’t want to have children, etc. Therefore, what intelligent man would want to get involved with American women? American women are generally immature, selfish, extremely arrogant and self-centered, mentally unstable, irresponsible, and highly unchaste. The behavior of most American women is utterly disgusting, to say the least. This blog is my attempt to explain why I feel American women are inferior to foreign women (non-American women), and why American men should boycott American women, and date/marry only foreign (non-American) women.

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  36. Liker veldig godt Ã¥ kikke innom bloggen din, men blogger ikke selv. Du tar sÃ¥ flotte bilder, at jeg svært gjerne kunne tenke meg at det blir du som tar bildene av konfirmanten vÃ¥r neste høst!Ha en spennende ventetid!…og JA, vil gjerne være med i trekningen!Nina:-)

  37. Vu là où est Monfils, ce n’est pas finale à Valence et Bercy qui le qualifie, mais victoire dans les deux cas, donc c’est hyper mal barré.Tipsa et Almagro, c’est au minimum finale à Bercy, et encore, à condition que soit Fish, soit Berdych soit battu au 1er tour.Delpo, ça le fait avec victoire à Valence et finale à Bercy (ou l’inverse).Bref je rejoins ta conclusion: sauf GROSSE surprise, c’est tout bon pour Tsonga, Fish et Berdych.

  38. Doesn’t surprise me a bit. Dirty old man living out his fantasies because he thinks he’s entitled to play around & hide behind his political office in safety. I hope they incarcerate him after they kick him out of congress!!!!!

  39. Hey Mark, it’s Guetty. We rode the filmmaker bus together driven by Maris. I love this video. My son Joakim loves science specifically biology and architecture. I think he needs to meet this mad scientist. Please send me more info about him. Take good care,GuettyP.S. Congrats on becoming a finalist. I love your project. I hope you get it.

  40. Som tam-resepti vaikuttaa tosi hyvältä, täytyy kokeilla (porkkanalla ja ilman rapuja!). Kasvisvaihtoehto se ei kyllä ole jos mukaan lorautetaan kalakstiketta, oletko kokeillut korvaavia kastikkeita? Vai muuttuuko salaatti silloin jo ihan joksikin muuksi kuin Som Tamiksi?

  41. Buon pranzo caro Ludo. Come!!, dici sempre che abbiamo una grande squadra e poi scrivi ” con il materiale che avevano a disposizione”.Continuo a non ritenerlo un grande allenatore….giusto riconoscer che ha fatto un buon lavoro ma stop. Per me colpa della sua presunzione ha frenato tante volte il Napoli.

  42. Google has launched a new tool within Google Webmaster Tools that allows Webmasters to create custom 404 pages. To access the tool, go to Google Webmaster Tools, then click on tools on the left and then click on “Enhance 404 pages.” You can then copy and paste the widget code…——————williamgeorge

  43. très bonne intervention avec des recentralisations sur les vrais problèmes, en sachant ne pas tomber dans le piège grossier de Steg qui tentait de caricaturer vos propos pour les décridibiliser.J’ai trouvé Alexandre Saubot très intéressant car pédagogique et posé

  44. Quando ero piccola a settembre passavamo giorni a cuocere, spellare a passare nel passa verdure quintali di pomodori (almeno a me sembravano tanti). Ognuno di noi figli aveva un compito nella catena di lavoro. Io, la più piccola, ero l’addetta a mettere la foglia i basilico nelle bottiglie.Tutto questo e il sapore della passata Panella, che ho avuto la fortuna di assaggiare, mi ha riportato alla mia infanzia, proprio come Ego!

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  46. Hi Charlie. How are you today;looking forward to seeing you again tomorrow (Friday) night for some nightime fun! Please be “dirty” for us and give us a good show as you usually do!Look forward to seeing your lovely tits (and the rest of your lovely body!)bouncing across my tv screen!Lots and lots of love as usualGeoffxxxxxx

  47. You are wrong that the USA has gotten nothing for the tribute they have paid the PLO. They are paying protection money so their airplanes and assets will not be attacked. This is most likely what was behind the Oslo treason as well. Aryeh ZelaskoBeit Shemesh

  48. BOYCOTT AMERICAN WOMEN! http://www.BoycottBitches.com I am an American man, and I have decided to boycott American women. In a nutshell, American women are the most likely to cheat on you, to divorce you, to get fat, to steal half of your money in the divorce courts, don’t know how to cook or clean, don’t want to have children, etc. Therefore, what intelligent man would want to get involved with American women? American women are generally immature, selfish, extremely arrogant and self-centered, mentally unstable, irresponsible, and highly unchaste. The behavior of most American women is utterly disgusting, to say the least. This blog is my attempt to explain why I feel American women are inferior to foreign women (non-American women), and why American men should boycott American women, and date/marry only foreign (non-American) women.

  49. I want to tell you about the modern crisis city level in sonic generations in the start off the level go back above and you will see 2 signs one of them wrote in it chao garden you can see them near the end of the level

  50. I’m still learning from you, while I’m making my way to the top as well. I absolutely enjoy reading everything that is written on your site.Keep the stories coming. I liked it!

  51. I apply for the job of making your business famous on the internet. I fix existing websites. I build new websites. My website work shows up on Google, Yahoo and Bing. The work I do for my business customers is commonly known as “web development optimization”. To test my skill-set, perform a nationwide search —- “web development optimization”. You will find my website organically positioned #1 throughout North America on Google, Yahoo and Bing. I have never paid any search engine company any money — ever! Sincerely,Net Media Markets

  52. Ph.HDésolé.Mais je n’ai rien inventé !L’indécence de ce communiqué pourrait relever d’une saillie intempestive d’humour noir, sur mon blog…Malheureusement… c’était bien sur Europe1.frMais Besson a peut-être trouvé, depuis, le moyen d’assurer le Le bal continue pendant les travaux« 

  53. A Windows Live-nál is elég csak egyszer bejelentkezni és használhatod az összes szolgáltatást: Spaces, SkyDrive, Hotmail, stb. Az már más kérdés, hogy például GMail helyett soha nem használnék Hotmailt, vagy a Spaces helyett szívesebben választanék más helyet blogolni…A Microsoft próbálja a Live szolgáltatásait bÅ‘víteni és népszerűsíteni, de még mindig fényévekre van a Googletól.

  54. Cindy, My Home Depot doesn’t have the grounding wire. I’m wondering if you have to buy the whole cable or just some bundles of the gauge you want.Lisa: I’ve used Extra thick embossing powder on baked clay and it worked fine. I don’t think I put 3 layers on though.

  55. salam doste azizin dovomin cmmente manedaneshjoye phd eghtesad dar iran hastam, sharayete pazhoheshi inam ham khubemodele arshadam 18eyek 2 hafteye pish holand budm kheili khosham omad.daneshgahe Delft reshteye man ya eshtehaye moshabeh baraye man dare?mituni rahnemaeim koni?mamnun misham

  56. Aino-Maria, kiitos viestistä ja kiva, että tykkäät! En voi luvata dekkarilistaa, koska en lue dekkareita, mutta sadepäivän lista on hyvä idea. Keksin jo heti pari mukavaa sadepäivän kirjaa. Listan ajankohta on kuitenkin varmaan vasta lähempänä kesää, nyt kun näyttää vielä siltä, että joudutaan aika kauan keksimään lukemista lumikinosten keskelle…Aakkoslukeminen taitaa olla nyt suorastaan trendikästä! 😉 Mutta idea on niin hyvä, ettei ihme, että se saa suosiota. Itse olen jo lukenut C:n, mutta en ole vielä kirjoittanut siitä. D on valitsematta.

  57. I love your blog.. very nice colors & theme. Did you create this website yourself or did you hire someone to do it for you? Plz answer back as I’m looking to design my own blog and would like to know where u got this from. appreciate it

  58. Jason, This definitely is valid. Melky now is playing the best baseball of his career though. It quite possibly could have more to do with his maturity than the city itself. But there is no denying that his numbers in KC and SF have been far better than they were in New York.

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  61. A ja bym chciał, żeby 724 jechało Śniadeckich, potem, żeby wybudowali nowy przejazd kolejowy i dalej Cyraneczki do Julianowskiej, żeby się dało na basen dojechać czy do Konstancina.

  62. Merci beaucoup Lili ! J’espère que tu en viendras vite à bout mais je n’en doute pas J’ai fait un milliard de sites pour trouver les boutons, jusqu’à ce que je tombe sur ceux-ci sur « A Little Mercerie »…J’ai vraiment halluciné sur les prix, certains étaient à 2€ pièce parfois !!

  63. It sounds to me like Glenda wants to ban teacher unions through some type of legislative process or act of Congress. How nice of Glenda to deny teachers their first amendment rights. This sounds quite undemocratic or as Glenda might say, just preposterous…REALLY !!

  64. / Dawson : Généralement le matin devant l’émission de Katie Couric.sur TF1 ça devient : Dawson : Non, ce n’est pas vrai Joey. Tu sais qu’on peut tout se dire. / Joey : Ah ouais ? Tu m’aimerais bien les cheveux éclaircis ? […]La même couleur que Jen, ça te plairait ?

  65. Love love the sleek and modern design, perfect for any interior!! Would love to give this to our second munchkin who is due in June, so being an interior design nut its perfect to be set up a couple of months ahead! Good luck to everyone!! And congrats on all the buns in the oven!!

  66. SERIOUSLY!!!!! This must be the most DIVINE sweet EVER!!!! :) :) :) Just had a piece hot and fresh out of the oven – it was just no way I was going to wait for it to cool down!!! WOW!!! Also, another great thing about these is that they're egg-free. I just know what I'm going to make for my dad when I go home to Norway for Christmas! He's allergic to eggs so he is not much of a cake eater but I am positive that he'd enjoy these tremedously! Thank you so much for sharing this recipe! Happy Thanksgiving. Best regards, Camilla

  67. Hello! I’m at work browsing your blog from my new apple iphone! Just wanted to say I love reading through your blog and look forward to all your posts! Carry on the great work!

  68. a ton avis wlad lmalah ga3 mlagtine c sa ?? ana malhi w je le connai w manabrihch aya win rah pbm ?? wili raha tochkor fih na3rafhom ga3 w ga3 khraj m3ahom voila

  69. Frumos gestul blondei Udrea pentru noi…alÅ£ii se laudă doar cu oraÅŸul nostru, dar habar n-au ce înseamnă redeschidrea Drumului Mare… e istorie scrisă modern ÅŸi binevenită…eu, cel puÅ£in, îi mulÅ£umesc doamnei deputat fiindcă ne-a ajutat ÅŸi sperăm s-o facă în continuare !

  70. Ett av de bästa blogginlägg jag läst på länge och beskriver även hur jag känner om Yes nya album (fast "Fragile" var min första Yesförälskelse).Lustigt sammanträffande att jag häromdagen funderade på att låta mina bekanta recensera progressiv rock från ett "ofördärvat" perspektiv.

  71. Iniziative come questa sono il viatico migliore per stare assieme, discutere di politica, ridare speranze ai Socialisti, ma soprattutto mettere in pratica gli insegamenti di coloro che hanno fatto grande l'Italia e dato dignità a chi si ritiene ancora Socialista. Io persoalmente non potrò essere presente a quel convegno, per me troppo lontano, ma vi sono vicino col cuore e col pensiero nella speranza che da tali incontri possa scaturire la possiblità di ritrovare tutti i socialisti sotto un'unica bandiera.Ciao

  72. Kochana księżniczko napewno odwiedzi Cię Wróżka Zębuszka:-):-):-). Mam nadzieję, że zakupy się udały:-). Może udało się kupić buciki?:-) Suuuper perełko, że tatuś jest teraz w domku i może odpocząć, a wolny czas spędzić na zabawie z tak cudowną córeczką jak Ty:-):-). Natusiu między kolorami tęczy rób kroki nieśmiałe i niech przyśnią Ci się rzeczy piękne i wspaniałe. Tęczowych snów:-):-)

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  74. Fan, har det redan gått fem år!? Jävlar vad fort tiden går asså!Stort GRATTIS på femårsdagen, TACK för alla underbara inlägg, AVGÅ för alla värdelösa analyser! Jag vet att ni kommer fortsätta PRESTERA i framtiden!WHO ARE WE?GLENN ARMY!

  75. Fairly insightful post. Never believed that it was this simple after all. I had spent a excellent deal of my time looking for someone to explain this subject clearly and you’re the only one that ever did that. Kudos to you! Keep it up

  76. Era un mos in tramvaiu 11(pe vremea cand locuiam impreuna eu si florentina:) )care ma ingrozea..il vedeam in fiecare zi, avea un palton de dimie, cum ar zice bunica-mea, vechi si rupt…si un baston..cu ala mai altoia cate un calator care nu vroia sa-i dea bani..si dupa ce facea turul tramvaiului, cand termina, incepea sa injure,ca nu-i da lumea bani..pe bune, mie imi era frica.

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  79. Wow! Great work, Kat!! I love the motion and the colors are awesome. Good for you for working big too. 16×20 lets you get into it. I’m so excited for you with this new direction. Love, love, love it!! As of now, I am signing mine on the back. I haven’t worked out what to do either Beautiful work – keep going – you are heading in a fabulous direction.

  80. Isopeikko,taidettiinpa kommentoida toistemme kirjoituksia ihan samaan aikaan. :)Itsehillintää, sitä minä tarvitsenkin, söin eilen liikaa herkkuja ja voin pahoin yöllä. Voi minua ahmattia! Ps. Joulukalenteri meni postissa lapsenlapselle, onneksi.

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