Anti-Caspase 3 antibody produced in rabbit

Product Name: Anti-Caspase 3 antibody produced in rabbit

Synonym: Anti-Apopain; Anti-CPP32; Anti-Yama

MDL Number: MFCD01321906
Product Type: Chemical

CAS NO: 1189986-59-1Toll-like Receptor (TLR) inhibitors
antibody Form: IgG fraction of antiserum
application(s)
microarray: suitable

western blot: 1:3,000 using Jurkat human T-cell leukemia cell extract
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: antigen mol wt 32 kDa
Shipped in: dry ice
species reactivity
human
Storage temp.: −20°C
Application: Anti-Caspase 3 antibody produced in rabbit is suitable for microarray and western blotting at a working dilution of 1:3000 using Jurkat human T-cell leukemia cell extract. It was used as a primary antibody for western blotting in a study to establish the biomolecular basis for the role of PCMT1 (isoaspartyl protein carboxyl-O-methyltransferase) as a part of a novel apoptosis regulatory mechanism. It was used to examine activation of caspase-3 via immunoblotting in a study to confirm the apoptosis of tumor cells mediated by AAV-ISN-T (recombinant adeno-associated virus vector with signal peptides of human insulin) administration. It was used as a primary antibody at 1:300 dilution for immunohistochemistry of deparaffinized sections of squamous cell carcinoma of the tongue containing surrounding normal tissue. It was used at a dilution of 1:1000 to detect caspase-3 and its cleaved Form P20 in a study.
Biochem/physiol Actions:
Caspase 3 cleaves many key proteins including poly(ADP-ribose) polymerase (PARP), sterol-regulatory element-binding proteins (SREBPs), DNA-dependent protein kinase (DNA-PK), α-fodrin, gelsolin, PKCd and DFF45/ICAD during apoptosis. High levels of this enzyme in lymphocytes suggest that it is an important mediator of apoptosis in the immune system. Deletion of CASP-3 gene in mice results in hyperplasia and cell abnormalities, indicating that caspase 3 is essential for morphogenetic cell death during normal brain development.
Features and Benefits:
Antibody Bioguarantee
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General description: Caspase 3 is a cytosolic protein present as a 32 kDa proenzyme in cells. It is activated by proteolytic cleavage into the 20 kDa (p20) and 11 kDa (p11) active subunits when cells undergo apoptosis.
Immunogen:
synthetic peptide corresponding to the N-terminal of human caspase 3 (amino acids 29-43 with C-terminally added lysine) conjugated to KLH. This sequence, unique to caspase 3, corresponds to N-terminal of the enzyme p20 subunit and is highly conserved in rat and mouse caspase 3.
Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: nwg
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/96/2/99