Product Name: Anti-phospho-CREB (pSer133) antibody produced in rabbit
MDL Number: MFCD01633981
Product Type: Chemical
CAS NO: 133868-46-9PPAR inhibitors
antibody Form: affinity isolated antibody
application(s)
immunoprecipitation: 1:50
western blot: 1:1,000 using extract from SK-N-MC cells, untreated or forskolin treated.
biological source
rabbit
clone
polyclonal
Conjugate: unconjugated
Form: buffered aqueous glycerol solution
Mol wt: antigen mol wt 43 kDa
Shipped in: wet ice
species reactivity
mouse, human, rat
Storage temp.: −20°C
Application: Anti-phospho-CREB (pSer133) antibody produced in rabbit may be used for immunoprecipitation at a working dilution of 1:50 and for immunoblotting at a working dilution of 1:1000 using an extract from SK-N-MC cells, untreated or forskolin treated. It was used for western blot analysis of proteins extracted from vascular smooth muscle cell cultures, separated by SDS-PAGE and blotted onto nitrocellulose membranes in a study. It was used to assess CREB activation by western blotting in a study.
Biochem/physiol Actions:
CREB is a phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Various extracellular signals including cAMP, membrane depolarization, increased intracellular Ca2+, and growth and neurotrophic factors stimulate this protein to activate transcription. Phosphorylation of CREB takes place at Ser133 and this regulates the ability of the protein to activate transcription. Studies show that CREB is a mediator in learning and long-term memory in both flies and mice.
Features and Benefits:
Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perForm in your application, we will issue a full credit or replacement antibody. Learn more.
Immunogen:
synthetic phosphopeptide corresponding to the region of human CREB that contains serine 133.
Physical Form: Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin, and 50% glycerol.
Specificity:
The antibody detects CREB when phosphorylated at Ser133 and phosphorylated ATF-1.
RIDADR: NONH for all modes of transport
WGK Germany: 2
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/95/2/117