Product Name: Casein Blocking Buffer
EC Number: 232-555-1
Product Type: Chemical
CAS NO: 38642-49-8CCR inhibitors
Form: powder blend
grade
for molecular biology
pH range
6.8 – 7.2 (reconstituted per directions)
solubility
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
Storage temp.: room temp
Application: Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.
Features and Benefits:
•Better blocking perFormance than dried milk or BSA
•Compatible with neutral and positively-charged membranes
•Compatible with chemiluminescent, colorimetric , and DIG detection assays
General description: Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.
Packaging:
1 L in poly bottle
Preparation Note: Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: room temp
UNSPSC
12161700
PubMed ID:http://jpet.aspetjournals.org/content/90/4/367