Product Name: Ciliary Neurotrophic Factor from rat
Synonym: CNTF
MDL Number: MFCD00211812
Product Type: Chemical
CAS NO: 329218-11-3Toll-like Receptor (TLR) inhibitors
Assay: ≥98% (SDS-PAGE)
Form: lyophilized powder
impurities
endotoxin, tested
Mol wt: mol wt 22.7 kDa
Recombinant: expressed in E. coli
Storage temp.: −20°C
suitability
suitable for cell culture
Analysis Note:
The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.
Biochem/physiol Actions:
Ciliary neurotrophic factor was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat ciliary neurotrophic factor share ~83% sequence homology and show cross-reactivity in bioactivity.
Physical Form: Lyophilized from a 0.2 μm filtered solution of 5 mM sodium acetate, pH 6.5.
RIDADR: NONH for all modes of transport
WGK Germany: 2
Purity: ≥98% (SDS-PAGE)
Storage Temp.: −20°C
UNSPSC
51111800
PubMed ID:http://jpet.aspetjournals.org/content/8/5/261