Product Name: Cystatin B human
Synonym: CPI-B; CST6; CSTB; EPM1; Liver Thiol Proteinase Inhibitor; PME; STFB; Stefin B
Product Type: Chemical
CAS NO: 1700663-41-7Tyrosinase inhibitors
description
contains a N-terminal 7X His-tag
Mol wt: apparent mol wt ~13 kDa by SDS-PAGE (reducing)
NCBI accession no.
NP_000091
Recombinant: expressed in E. coli
Storage temp.: −20°C
Application: Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.
Biochem/physiol Actions:
Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L Forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain.
General description: Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).
Reconstitution:
Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352202
PubMed ID:http://jpet.aspetjournals.org/content/80/4/386