Product Name: Monoclonal Anti-Cdk4 antibody produced in mouse
MDL Number: MFCD00282839
Product Type: Chemical
CAS NO: 1351926-90-3Neuronal Signaling inhibitors
antibody Form: ascites fluid
application(s)
immunocytochemistry: suitable
immunoprecipitation: suitable
microarray: suitable
western blot: 1:1,000 using a cultured human tumor cell line extract
biological source
mouse
clone
DCS-31, monoclonal
Conjugate: unconjugated
contains
15 mM sodium azide
isotype
IgG2a
Mol wt: antigen mol wt 33 kDa
Shipped in: dry ice
species reactivity
mouse, human, rat
Storage temp.: −20°C
Application: Monoclonal Anti-Cdk4 antibody was used in:
•immunoprecipitation of human melanoma cell line.
•immunofluorescence of fibrosarcoma cells.
•western blot analysis of Notch1 protein. The antibody is also suitable for microarray and western blot at a dilution of 1:1,000 by using a cultured human tumor cell line extract.
Biochem/physiol Actions:
In association with cyclins, cyclin-dependent kinases (CDKs) Forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.
General description: Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
Immunogen:
recombinant human Cdk4 protein
Specificity:
The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/93/1/18