S of TNF IL10, and of your suppressor of cytokine signalling SOCS-3 had been JD-5037 site detected and quantified by competitive RT-PCR and by Western blot. DNA binding activity on the transcription factors NF-B and STAT-3 was detected by eletrophoretic-mobility-shift assay (EMSA) and super shift, and phosphorylation of IB- and STAT-3 by Western blot. CellularP148 Methylprednisolone sodium succinate reduces postoperative hyperthermia but doesn’t have an effect on cardiac function just after aortic valve replacementR Jara-Rubio*, J Galcer?, E Serrano, J Diaz, LF Carbonell *Intensive Care Unit, Hospital Universitario Arrixaca, 30120 Murcia, Spain; Departamento de Fisiolog , Universidad de Murcia, Spain Objective: Steroids have been utilised in cardiac surgery for a lot of years to lessen the inflammatory response related with extracorporeal circulation. On the other hand, their clinical advantages have not been properly established. The aim in the present study was to evaluate the impact of methylprednisolone on body temperature and cardiac index within the postoperative period of aortic valve replacement with extracorporeal circulation and aortic clamp. Patients: Twenty-two consecutive individuals undergoing aortic valve replacement and extracorporeal circulation. The sufferers received methylprednisolone (30 mg/kg; n = 11, MP group) or received no medication (n = 11, no MP group) just before cardiopulmonary bypass. Clinical and demographic characteristics of your individuals have been comparable in both groups.Available on-line http://ccforum.com/supplements/6/SFigureMP37.09 37.FigureNo MP37.MPNo MPCI (l/min/m2)36.63 35.T36 3535.four Hours4 Hours4 Hours4 HoursMeasurements and most important outcomes: Body temperature (To, oC) and cardiac index (CI, l/min/m2) were measured inside the postoperative period on admission within the ICU and at 4 hours and 8 hours immediately after ICU admission. On admission to ICU there were no differences in temperature among groups. Body temperature was drastically lower in group MP at 4, and 8 hours after ICU admission (P < 0.05) (Fig. 1).Cardiac index increased during the postopera-tive period, with no significant differences between groups (Fig. 2). Conclusion: Methylprednisolone sodium succinate reduces hyperthermia without affecting cardiac function in the postoperative period of aortic valve replacement with extracorporeal circulation and aortic clamp.P149 Hemostatic and fibrinolysis markers in serum and shed mediastinal blood after elective coronary artery bypass graftingAA Peivandi*, M Dahm*, D Peetz, W Kasper-Koenig*, H Oelert* *Department of Cardiothoracic and Vascular Surgery, and Institute of Clinical Chemistry, University Hospital Mainz, D-55131 Mainz, Germany Objective: Reduction of homologous blood products in cardiac surgery is mainly achieved by autologous blood salvage. One of the most customary methods consists in autotransfusion of shed mediastinal blood within the first 6 hours after surgery. Aim of this prospective study was to compare serum and shed mediastinal blood qualities of hemostatic and fibrinolysis markers early after elective coronary artery bypass grafting (CABG). Methods: Forty-seven patients (mean age 68.1 ?6.9, 15 female/ 32 male) underwent first-time elective CABG with extracorporal circulation via median sternotomy. Activated partial thromboplastin time (aPTT), prothrombin time (Quick's value), international normalized ratio (INR), thrombin time, and fibrinogen (factor I) in arterialTable PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20724694 1 Soon after admission for the ICU Shed blood Quick’s worth ( ) INR APTT (s) Thrombin time (s).