Epresentative of OsGRF4 promoter haplotypes A, B and C (see main text) are shown. e, OsGRF4 mRNA abundance in many rice varieties under the high N situations (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance information is all relative for the abundance of rice Actin2 mRNA. Information shown as mean s.e.m. (n = 3). Unique letters denote significant variations (P 0.05, Duncan’s 1-Aminocyclopropane-1-carboxylic acid Protocol numerous variety test). f, Comparisons of OsGRF4 mRNA abundance in selected rice varieties grown in in between high (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N situations. Information shown as mean s.e.m. (n = 3). Abundance information is all relative to that in HN (set to 1). P 0.05 as in comparison to HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 members of the family in NJ6 plants grown at diverse levels of N provide (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N conditions (set to one). Data shown as imply s.e.m. (n = three). Different letters denote important differences (P 0.05, Duncan’s a number of range test).Europe PMC Funders Cangrelor (tetrasodium) GPCR/G Protein Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; obtainable in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure two. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Mature plant height. Information shown as mean s.e.m. (n = 16). Various letters denote substantial differences (P 0.05, Duncan’s numerous range test). b, The amount of tillers per plant. c, The number of grains per panicle. Information shown as mean s.e.m. (n = 16). Distinctive letters denote significant differences (P 0.05, Duncan’s numerous range test). d, Flag-leaf width. Information shown as mean s.e.m. (n = 16). Unique letters denote substantial differences (P 0.05, Duncan’s multiple variety test). e, Culm (stem) width expressed as diameter in the uppermost internode. Data shown as mean s.e.m. (n = 16). Diverse letters denote substantial differences (P 0.05, Duncan’s multiple variety test). f, Grain yield per plant. Data shown as imply s.e.m. (n = 220). Distinctive letters denote substantial variations (P 0.05, Duncan’s numerous variety test). g, Relative root abundance of OsAMT1.2 mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Information shown as mean s.e.m. (n = 3). Different letters denote considerable differences (P 0.05, Duncan’sNature. Author manuscript; out there in PMC 2019 February 15.Li et al.Pagemultiple range test). h, Root glutamine synthase (GS) activities. Information shown as imply s.e.m. (n = three). Different letters denote significant differences (P 0.05, Duncan’s many variety test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Data shown as mean s.e.m. (n = 3). Unique letters denote important differences (P 0.05, Duncan’s many range test). j, Shoot glutamine synthase (GS) activities. Information shown as imply s.e.m. (n = 3). Diverse letters denote considerable variations (P 0.05, Duncan’s a number of range test). k-n, Flag-OsGRF4 mediated ChIP-PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.