BzATP (triethylammonium salt) manufacturer Nuscript; available in PMC 2019 February 15.Li et al.Pageco-transformed into tobacco leaf epidermal cells with each other with a construct expressing SLR1 tagged with all the Eliglustat medchemexpress C-terminus of YFP. Scale bar, 60 m. The photos of BiFC assays represent among the three experiments performed independently with comparable outcomes (panels b and c).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure 6. SLR1 inhibits OsGRF4-OsGIF1 self-promotion of OsGRF4 mRNA and OsGRF4 protein abundance.a, OsGFR4 mRNA abundance, plant genotypes as indicated. Abundance shown relative to that in NJ6 (=1). Information shown as mean s.e.m. (n = 3). Distinctive letters denote considerable differences (P 0.05, Duncan’s many variety test). b, The effects of GA and PAC on OsGRF4 mRNA abundance in 2-week-old NJ6 plants. Abundance shown relative to that in water treatment handle (=1). Data shown as imply s.e.m. (n = three). Distinct letters denote substantial differences (P 0.05, Duncan’s multiple range test). c, ChIP-PCR OsGRF4mediated enrichment (relative to input) of GCGG-containing OsGRF4 promoter fragments (marked with ). Data shown as imply s.e.m. (n = 3). Unique letters denote important variations (P 0.05, Duncan’s various range test). d, OsGRF4-activated promotion of transcription from the OsGRF4 gene promoter-luciferase reporter construct is enhanced byNature. Author manuscript; readily available in PMC 2019 February 15.Li et al.PageOsGIF1 and inhibited by SLR1. Abundance of LUCREN shown relative to that in empty vector handle (=1). Information shown as mean s.e.m. (n = 3). Diverse letters denote important differences (P 0.05, Duncan’s a number of range test). e, OsGRF4 abundance (as detected by an anti-OsGRF4 antibody), plant genotypes as indicated. HSP90 serves as loading handle. The images of western blots represent among the three experiments performed independently with equivalent outcomes.Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure 7. The OsGRF4-SLR1 antagonism regulates carbon assimilation and plant growth.a, b, Relative shoot abundances of C-fixation gene mRNAs. Abundances of transcripts of genes regulating photosynthesis (a), sucrose metabolism and transportphloem loading (b) in NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 plants. Data shown as mean s.e.m. (n = three). Abundances in NJ6 and NJ6-sd1-OsGRF4ngr2 expressed relative to NJ6-sd1 (= 1). Different letters denote significant differences (P 0.05, Duncan’s several variety test). c, ChIP-PCR assays. Diagram depicts the OsPsbS1, OsTPS1 and OsSWEET11 promoters and regions employed for ChIP-PCR, and GCGG-containing promoter fragment (marked with ) enrichment (relative to input). Information shown as mean s.e.m. (n = 3). Unique letters denote substantial differences (P 0.05, Duncan’s many range test). d, Transactivation assays. The LUCREN activity obtained from a co-transfection with an empty effector construct andNature. Author manuscript; obtainable in PMC 2019 February 15.Li et al.Pageindicated reporter constructs was set to become one particular. Data shown as imply s.e.m. (n = 9). Various letters denote significant variations (P 0.05, Duncan’s various variety test). e, Immunoblot detection of OsLhca1, OsLhca3, OsLhca4, OsLhcb2, OsPsaD and OsPsaE employing antibodies as shown in genotypes as indicated. HSP90 serves as loading control. The photos of western blots represent among the three experiments performed independently with related outcomes. f-i, Comparisons.