From the FCT and also the FSE, below the POCI system. F.
From the FCT as well as the FSE, under the POCI program. F. Coutinho received a analysis contract beneath the FCT project POCI-01-0145-FEDER-029540 (PTDC/ BIAOUT/29540/2017). A. Couto and P. Enes have scientific employment contracts that are supported by national funds by means of the FCT. The authors would also like to thank BUGGYPOWER for the type supply in the Nannochloropsis gaditana biomass applied in this study. Conflicts of Interest: The authors declare no conflict of interest.(1)Mar. Drugs 2021, 19,17 of
marine drugsArticleA Novel Angiotensin-I-Converting Enzyme (ACE) Goralatide Purity & Documentation Inhibitory Peptide from Takifugu flavidusYongchang Su 1,2, , Shicheng Chen 3, , Shuilin Cai 1,two , Shuji Liu two , Nan Pan 2 , Jie Su two , Kun Qiao two , Min Xu 2 , Bei Chen two , Suping Yang 1, and Zhiyu Liu 2, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; [email protected] (Y.S.); [email protected] (S.C.) Crucial Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Analysis Bafilomycin C1 Description Institute of Fujian, Xiamen 361013, China; [email protected] (S.L.); [email protected] (N.P.); [email protected] (J.S.); [email protected] (K.Q.); [email protected] (M.X.); [email protected] (B.C.) Division of Clinical and Diagnostic Sciences, College of Health Sciences, Oakland University, 433 Meadowbrook Road, Rochester, MI 48309, USA; [email protected] Correspondence: [email protected] (S.Y.); [email protected] (Z.L.) These authors contributed equally to this perform.Citation: Su, Y.; Chen, S.; Cai, S.; Liu, S.; Pan, N.; Su, J.; Qiao, K.; Xu, M.; Chen, B.; Yang, S.; et al. A Novel Angiotensin-I-Converting Enzyme (ACE) Inhibitory Peptide from Takifugu flavidus. Mar. Drugs 2021, 19, 651. https://doi.org/10.3390/ md19120651 Academic Editor: Marialuisa Menna Received: 21 October 2021 Accepted: 19 November 2021 Published: 23 NovemberAbstract: Alcalase, neutral protease, and pepsin were applied to hydrolyze the skin of Takifugu flavidus. The T. flavidus hydrolysates (TFHs) with the maximum degree of hydrolysis (DH) and angiotensin-Iconverting enzyme (ACE)-inhibitory activity have been chosen and then ultra-filtered to acquire fractions with components of unique molecular weights (MWs) (1, 1, 30, one hundred, and 50 kDa). The elements with MWs 1 kDa showed the strongest ACE-inhibitory activity using a half-maximal inhibitory concentration (IC50 ) of 0.58 mg/mL. Purification and identification working with semi-preparative liquid chromatography, Sephadex G-15 gel chromatography, RP-HPLC, and LC S/MS yielded 1 new possible ACE-inhibitory peptide, PPLLFAAL (non-competitive suppression mode; IC50 of 28 mol -1 ). Molecular docking and molecular dynamics simulations indicated that the peptides must bind well to ACE and interact with amino acid residues as well as the zinc ion at the ACE active internet site. In addition, a short-term assay of antihypertensive activity in spontaneously hypertensive rats (SHRs) revealed that PPLLFAAL could substantially reduce the systolic blood pressure (SBP) and diastolic blood stress (DBP) of SHRs following intravenous administration. These benefits recommended that PPLLFAAL might have prospective applications in functional foods or pharmaceuticals as an antihypertensive agent. Keyword phrases: Takifugu flavidus; hydrolysis; ACE-inhibitory activity; purification and identification; molecular docking; antihypertensive activityPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional a.