Involved in neurodegenerative and cerebrovascular diseases and for their feasible use as clinical biomarkers. Funding: Italian Ministry of Overall health, Ricerca corrente 2017018.OWP2.03 = PS04.Microscale electrophoretic separations of exosomes Yuliya Shakalisava; Delaram Zahouri; Roy Kreekel; Thomas Hankemeier Leiden Academic Center for Drug Investigation, Leiden University, Leiden, The NetherlandsOWP2.02 = PS05.Detection and characterization of various neuronal and glial populations of exosomes by surface CD158d/KIR2DL4 Proteins Gene ID plasmon resonance imaging Silvia Picciolini1; Alice Gualerzi1; Andrea Sguassero1; Furio Gramatica1; Massimo Masserini2; Marzia Bedoni1 Laboratory of Nanomedicine and Clinical Biophotonics (LABION), IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy; 2Nanomedicine Center NANOMIB, School of Medicine and Surgery, University of Milano-Bicocca, Monza, ItalyBackground: The usage of exosomes for diagnostic and disease monitoring purposes is becoming especially appealing, taking into consideration that the pathological status greatly impacts the exosomes content. Moreover, brainderived exosomes present in blood plasma could be noticed as a directBackground: Exosomes have gathered interest due to their diagnostic and therapeutic possible. They are present in blood, urine and saliva, which make them an appealing resource for non-invasive etiological and diagnostic analysis. Undoubtedly, size and optical properties would be the most studied, which can be reflected in the present isolation approaches dominating the analysis field. Our analysis makes a contribution to further investigation of electrophoretic properties of exosomes. For the very first time we report a microscale separation system capillary electrophoresis (CE) for characterisation of exosomes. The aim was to further explore electrophoretic behaviour of exosomes and investigate the electrophoretic signatures of exosomes in CE format. Procedures: CE was employed to study the electrophoretic migration of requirements of exosomes inside the narrow bore capillary below the electric field. Laser-induced fluorescent detector was utilized and various fluorescent markers were investigated for labelling of exosomes. Capillary zone electrophoresis (CZE) and capillary isotachophoresis(cITP) modes of CE had been used in this study. To improve the resolution of exosomal fractions in cITP mode, a variety of spacer compounds were investigated. The method was applied towards the human exosomes samples. Outcomes: The numerous zones of exosomes can be noticed in the electropherogram of exosomes requirements. These indicate the subpopulations of exosomes inside the commercial sample of purified exosomes. These subpopulations show differences in their electrophoretic mobility that are according to their size and charge properties. Various fluorescent markers supplied an informative insight in to the migration of distinct fractions ofISEV 2018 abstract bookexosomes depending on the mechanisms of labelling. cITP approach was superior to CZE in terms of sensitivity and resolution. The evaluation of human exosomes samples revealed exceptional signatures of exosomal fractions. The results on the healthier vs illness samples are going to be presented. Summary/conclusion: Electrophoretic signatures of exosomes had been effectively investigated in CE format. Electrophoretic properties of exosomes can supply an insightful technique of characterization. Funding: This project has received MMP-3 Proteins Gene ID Funding from the European Union’s Horizon 2020 analysis and innovation programme below grant agreement No 709077 Marie Sklodo.