Way have been identified by KEGG enrichment annotation (Fig. 4: five). KEGG analysis showed
Way had been identified by KEGG enrichment annotation (Fig. four: five). KEGG evaluation showed that compared with CAK (BR MAPK13 site spraying for 0 h), the expression in the UTPglucose-1-phosphate uridylyltransferase (UGP), SPS, glucose-6-phosphate isomerase (GPI), pyrophosphateJin et al. BMC Genomics(2022) 23:Web page ten ofFig. 5 A probable model of the BR signaling pathway with BRs (the activation state of BR signaling) sprayed onto tea leavesJin et al. BMC Genomics(2022) 23:Page 11 offructose-6-phosphate 1-phosphotransferase (PFP), and epidermis-specific secreted glycoprotein (EP) key regulatory genes related towards the sucrose biosynthesis pathway had been upregulated after BR spraying for 3 h, 9 h, 24 h, and 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes in the biosynthetic pathway of flavonoidsEleven genes involved in flavonoid biosynthesis have been identified by KEGG enrichment annotation (Fig. 4: six). The flavonoid biosynthesis-related genes PAL, C4H, 4CL, eIF4 Molecular Weight chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3,5-hydroxylase (F3’5’H), DFR, LAR, ANR, and UFGT have been upregulated, with peak values observed at 48 h.DiscussionBR signal transduction mechanism in tea leavesThrough KEGG enrichment and annotation, 26 genes involved inside the BR signal transduction pathway had been identified. In accordance with the heat maps of genes related to BR signal transduction beneath unique BR therapies, it was located that 26 genes inside the BR signal transduction pathway had been considerably upregulated with escalating BR spraying time. Combined with the BR signal transduction maps of Arabidopsis and rice, we describe a doable model in the BR signal pathway in tea leaves [291] (Fig. five). At present, the signal transduction pathway of BR in Arabidopsis and rice has been reported. Compared with rice, the signal transduction pathway of BR in tea leaves is related to that of Arabidopsis [24]. As opposed to the BR signal transduction pathway within a. thaliana, BAK1-like kinase contains each SERK and TMK4 in the BR signal transduction pathway of tea leaves. In our transcriptome data, the ATBS1-interacting things (AIF) and paclobutrazol resistance 1 (PRE) genes didn’t significantly differ in expression levels, whereas that of your teosinte branched (TCP) gene was important. AIF is the negative regulator of BR signal transduction, though PRE and TCP will be the optimistic regulators of BR signal transduction [34]. The results showed that TCP, the forward regulator of BR signal transduction, plays a major role within the effects of your exogenous spraying of BRs onto young tea leaves.Exogenous spraying of BR promotes the growth and improvement of tea plantsGBSS, and SBE genes associated to starch synthesis; along with the flavonoid biosynthesis-related PAL, C4H, 4CL, CHS, CHI, F3H, F3’5, DFR, LAR, ANR, and UFGT genes were identified. The outcomes showed that exogenous spraying of BRs upregulated the expression of genes connected to sucrose synthesis, chlorophyll synthesis, starch synthesis, and flavonoid biosynthesis. It may be inferred that exogenous BR spraying increased the content of sucrose, chlorophyll, starch, and flavonoids. Furthermore, a big number of extremely expressed cyclin genes, such as Cyc, CycD3, CycD4, and CDC6, had been identified. Cell cycle regulatory proteins can bind to cell differentiation cycle-coding proteins and activate corresponding protein kinases, as a result promoting cell division. BRs can improve plant development by advertising cell division.